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Muller-Kauffmann Tetrathionate Novobiocin Broth

suitable for microbiology, NutriSelect® Plus

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NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

brilliant green, 0.0095 g/L
calcium carbonate, 38.7 g/L
Casein enzymic hydrolysate, 8.6 g/L
ox bile, 4.75 g/L
peptic digest of animal tissue, 4.3 g/L
sodium chloride, 2.6 g/L
sodium thiosulphate, pentahydrate, 47.8 g/mL

manufacturer/tradename

NutriSelect® Plus

final pH

8.2±0.2 (25 °C)

application(s)

environmental
food and beverages
sample preparation

microbiology

suitability

Salmonella spp.

Related Categories

Application

Mueller Kauffman Tetrathionate Novobiocin Broth is used for improved enrichment and isolation of Salmonellae.

Preparation Note

Suspend 89.42 grams of dehydrated mediumI in 1000 ml distilled water. Heat the medium just to boiling. DO NOT AUTOCLAVE. Cool to 45-50°C and just before use aseptically add 20 ml of iodine solution (20 gram iodine and 25 gram potassium iodide in 100 ml sterile distilled water) along with rehydrated contents of 1 vial of MKTT Novobiocin Supplement (Cat. No. 79894). Mix well to disperse calcium carbonate uniformly before dispensing in sterile tubes.
Note: Due to presence of calcium carbonate, the prepared media forms opalescent solution with white precipitate.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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