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SBR00025

Sigma-Aldrich

Novobiocin Ready Made Solution

100 mg/mL in water

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Synonym(s):
Novobiocin Ready Made Solution
Linear Formula:
C31H35N2NaO11
Molecular Weight:
634.61

biological source

microbial

assay

≥90%

form

liquid

concentration

100 mg/mL in water

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
neoplastics

mode of action

DNA synthesis | interferes
enzyme | inhibits

storage temp.

−20°C

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Novobiocin Ready Made Solution 100 mg/mL in water

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antibiotic activity spectrum

Gram-negative bacteria, mycobacteria, Gram-positive bacteria, neoplastics

antibiotic activity spectrum

-

antibiotic activity spectrum

-

antibiotic activity spectrum

-

assay

≥90%

assay

-

assay

-

assay

≥99% (HPLC)

form

liquid

form

-

form

liquid

form

DMSO solution

biological source

microbial

biological source

-

biological source

-

biological source

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

General description

Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic commonly derived from Streptomyces Sp. It exerts its antibacterial activity by inhibiting Topo II α, which corresponds to bacterial DNA gyrase. It is effective against both gram-positive and gram-negative bacteria and is significant in research involving highly beta-lactam-resistant pneumococcal infections and anti-orthopoxvirus applications. Functioning as a competitive inhibitor in the ATPase reaction catalyzed by GyrB, Novobiocin is employed in microbiology and cell biology research to effectively prevent the growth of bacterial contaminants.

Application

Novobiocin has been used:
  • in cell culture systems to treat mycoplasma contaminations.[3]
  • in a research to where it has exhibited anti-cancer effects by inhibition of Hsp90 (heat shock protein)[4],[5]

Biochem/physiol Actions

Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II

Activity spectrum: Active against gram-positive and gram-negative bacteria

Features and Benefits

  • Ready available solution reduce the need for preparation time
  • Commonly used in Cell Biology and Biochemical applications
  • High quality antibiotic suitable for mulitple research applications

Preparation Note

Novobiocin Ready Made Solution is light sensitive. Thaw this product at room temperature. It is recommended to avoid repeated freeze thaw cycles of this product. The suggested concentration of Novobiocin for cell culture assays is 50-1000 μg/mL,,, therefore, it is recommended to dilute the Ready Made Solution 1:100-2000.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mycoplasmas in tissue culture.
Macpherson, I.
Journal of Cell Science, 1, 145-168 (1966)
Synthesis and biological evaluation of arylated novobiocin analogs as Hsp90 inhibitors.
Kusuma, B. R., Duerfeldt, A. S., & Blagg, B. S.
Bioorganic & Medicinal Chemistry Letters, 21, 7170-7174 (2011)
Environmentally constrained mutation and adaptive evolution in Salmonella.
Massey, R. C., Rainey, P. B., Sheehan, B. J., Keane, O. M., & Dorman, C. J.
Current Biology, 9, 1477-1481 (1999)
Effects of novobiocin, coumermycin A1, clorobiocin, and their analogs on Escherichia coli DNA gyrase and bacterial growth.
Hooper, D. C., Wolfson, J. S., McHugh, G. L., Winters, M. B., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 22, 662-671 (1982)
Novobiocin decreases SMYD3 expression and inhibits the migration of MDA-MB-231 human breast cancer cells.
Luo, X. G., Zou, J. N., Wang, S. Z., Zhang, T. C., & Xi, T.
IUBMB Life, 62, 194-199 null

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