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97622

Supelco

2-Mercaptoethanol

for HPLC derivatization, LiChropur, ≥99.0% (GC)

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Synonym(s):
β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
Linear Formula:
HSCH2CH2OH
CAS Number:
Molecular Weight:
78.13
Beilstein/REAXYS Number:
773648
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.05

grade

for HPLC derivatization

Quality Level

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

assay

≥99.0% (GC)

form

liquid

quality

LiChropur

expl. lim.

18 %

concentration

14.3 M (pure liquid)

technique(s)

HPLC: suitable

refractive index

n20/D 1.500 (lit.)
n20/D 1.500-1.502

bp

157 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

storage temp.

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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1 of 4

This Item
63689M3148444203
vibrant-m

97622

2-Mercaptoethanol

-
vibrant-m

63689

2-Mercaptoethanol

Premium Grade
vibrant-m

M3148

2-Mercaptoethanol

Essential+ Grade
technique(s)

HPLC: suitable

technique(s)

RNA extraction: suitable, protein extraction: suitable

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

technique(s)

-

assay

≥99.0% (GC)

assay

≥99.0% (GC)

assay

99% (GC/titration)

assay

≥99% (GC)

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

15-25°C

form

liquid

form

liquid

form

liquid

form

liquid

General description

2-Mercaptoethanol is a thiol compound showing growth promoting properties on mouse lymphoma L1210 cells in vitro. It also enhances viability, blast transformation and antibody formation in lymphocyte cultures.

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
suitable for derivatisation of Amine groups

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Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Storage Class

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup


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Mechanism of growth stimulation of L1210 cells by 2-mercaptoethanol in vitro. Role of the mixed disulfide of 2-mercaptoethanol and cysteine
Ishii T, et al.
The Journal of Biological Chemistry, 256(23), 12387-12392 (1981)
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