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β-Mercaptoethanol, Molecular Biology Grade

A disulfide-reducing agent.

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Synonym(s):
2-Mercaptoethanol, BME, β-Mercaptoethanol, 2-Hydroxyethylmercaptan, Thioethylene glycol
Linear Formula:
HSCH2CH2OH
CAS Number:
Molecular Weight:
78.13
Beilstein/REAXYS Number:
773648
MDL number:
UNSPSC Code:
12161504
NACRES:
NA.25

grade

Molecular Biology

Quality Level

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

assay

≥99% (GC)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

concentration

14.3 M (pure liquid)

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

mp

<-50 °C

density

1.12 g/cm3 at 20 °C
1.114 g/mL at 25 °C (lit.)

cation traces

heavy metals (as Pb): ≤0.0001%

absorption

≤0.31 at 280 nm
≤1.50 at 260 nm

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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General description

A disulfide-reducing agent.
A disulfide-reducing agent. Reagent commonly used in organic synthesis (aborsorbance: ≤1.50 at 260 nm; ≤0.31 at 280 nm).

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Warning

Toxicity: Toxic (F)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

wgk_germany

WGK 3

flash_point_f

165.2 °F

flash_point_c

74 °C


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