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B0126

Sigma-Aldrich

Bromophenol Blue

titration: suitable

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Synonym(s):
3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form
Empirical Formula (Hill Notation):
C19H10Br4O5S
CAS Number:
Molecular Weight:
669.96
Beilstein/REAXYS Number:
61698
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

agency

suitable for SM 2310
suitable for SM 2320

Quality Level

form

powder

technique(s)

titration: suitable

color

faint beige to very dark beige
faint orange to very dark orange

visual transition interval

3.0-4.6, yellow-green to blue

mp

273 °C

solubility

methanol: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

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1 of 4

This Item
1143913187441.08122
Bromophenol Blue titration: suitable

B0126

Bromophenol Blue

Bromophenol Blue ACS reagent

114391

Bromophenol Blue

Bromophenol Blue solution 0.04 wt. % in H2O

318744

Bromophenol Blue solution

Bromophenol blue indicator ACS,Reag. Ph Eur

1.08122

Bromophenol blue

color

faint beige to very dark beige, faint orange to very dark orange

color

-

color

-

color

-

solubility

methanol: 10 mg/mL

solubility

ethanol: 0.1%

solubility

-

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

form

powder

form

powder

form

liquid

form

solid

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

2-30°C

General description

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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