X4126

Xylene Cyanol FF

BioReagent, Molecular Biology

• Synonym(s): Acid blue 147, Cyanol FF, XC
• Empirical Formula (Hill Notation): C₂₅H₂₇N₂NaO₆S₂
• CAS Number: 2650-17-1
• Molecular Weight: 538.61
• Colour Index Number: 42135
• EC Number: 220-167-5
• MDL number: MFCD00019481
• UNSPSC Code: 12171500
• PubChem Substance ID: 57654732
• NACRES: NA.52

Properties

• grade: Molecular Biology
• Quality Level: 200
• product line: BioReagent
• form: powder
• technique(s): nucleic acid detection: suitable
• solubility: H₂O: complete 1 mg/mL, clear, blue
• suitability: suitable for nucleic acid staining
• storage temp.: room temp
• SMILES string: [Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O
• InChI: 1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;

Description

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Xylene Cyanol FF is used as a:

• histological stain and as tracker dye in DNA sequencing and electrophoresis[1][2]
• gel-loading dye[3]
• formamide RNA loading dye[4]
• tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer[5]

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves