Skip to Content
MilliporeSigma
All Photos(3)

Documents

O3756

Sigma-Aldrich

Orange G

for NA electrophoresis

Synonym(s):

1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, Acid Orange 10, Wool Orange 2G

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Empirical Formula (Hill Notation):
C16H10N2Na2O7S2
CAS Number:
1936-15-8
Molecular Weight:
452.37
Colour Index Number:
16230
Beilstein/REAXYS Number:
4120705
EC Number:
217-705-6
MDL number:
MFCD00012457
UNSPSC Code:
12171500
PubChem Substance ID:
57654483
NACRES:
NA.52

grade

for molecular biology

form

powder

technique(s)

nucleic acid detection: suitable

solubility

water: 1 mg/mL, clear, orange to red

suitability

suitable for gel electrophoresis

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;

InChI key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

Looking for similar products? Visit Product Comparison Guide

General description

Orange G is an azo dye that is acidic in nature. It is widely used as a tracking dye for nucleic acid gel electrophoresis to track DNA front in agarose gels. Typically runs at 50bp in up to 2.5% agarose.

Application

Orange G has been used as a DNA gel loading dye.
Orange G is a tracking dye used in nucleic acid electrophoresis to track DNA front in agarose gels. Also used in SDS-capillary electrophoresis as a standard. Typically runs at 50bp in up to 2.5% agarose.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Degradation of azo dye Orange G in aqueous solutions by persulfate with ferrous ion
Xu XR and Li xZ
Separation and Purification Technology, 72(1), 105-111 (2010)
A Hiraoka et al.
Journal of chromatography. B, Biomedical sciences and applications, 697(1-2), 141-147 (1997-10-29)
Proteins of low molecular (M(r)) in human cerebrospinal fluid (CSF) were analyzed by capillary electrophoresis in a sodium dodecyl sulfate-containing polymer solution. Under the conditions employed, peaks of beta 2-microglobulin (beta MG) (M(r): 11,700), gamma-trace protein (12,300), myelin basic protein
P C Dabas et al.
Journal of capillary electrophoresis, 4(5), 253-256 (1998-09-02)
A simple and reproducible micellar electrokinetic chromatography (MEKC) method has been developed for the quantitation of suramin serum levels to be used for its therapeutic drug monitoring (TDM). A running buffer solution of 20 mM sodium borate, 75 mM sodium
Differential salt fractionation of nuclei to analyze chromatin-associated proteins from cultured mammalian cells
Herrmann C, et al.
Bio-protocol, 7(6), 1986-1986 (2017)
Touchdown PCR for increased specificity and sensitivity in PCR amplification
Korbie DJ and Mattick JS
Nature Protocols, 3(9), 1452-1452 (2008)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service