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Key Documents

B3269

Sigma-Aldrich

Bromphenol Blue–Xylene Cyanole Dye solution

lyophilized powder

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

product name

Bromphenol Blue–Xylene Cyanole Dye solution, lyophilized powder

form

lyophilized powder

Quality Level

color

dark blue

pH range

8-8.6

solubility

water: 0.2 vial/mL, blue

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

General description

Bromophenol Blue–Xylene Cyanol Dye solution is a tracking dye for nucleic acid electrophoresis in agarose or polyacrylamide gels including DNA sequencing.

Application

Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.

Reconstitution

Add 5 mL water to the vial to dissolve. Reconstituted solution contains 0.5% bromphenol blue and 0.5% xylene cyanole. Dilute 1:10 for nucleic acid electrophoresis in agarose or polyacrylamide gels or add 4 parts formamide to 1 part dye solution for DNA sequencing.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges


Certificates of Analysis (COA)

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Genomic sequencing.
Church GM and Walter G
Proceedings of the National Academy of Sciences of the USA, 81.7, 1991-1995 (1984)
CLIP: construction of cDNA libraries for high-throughput sequencing from RNAs cross-linked to proteins in vivo.
Wang Z, et al.
Methods, 48, 287-293 (2009)

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