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B2551

Millipore

Brewer thioglycollate medium

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
Thioglycollate Medium (Brewer)
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

pH

7.2±0.2 (25 °C)

application(s)

clinical testing
food and beverages
sterility testing

microbiology

suitability

anaerobic bacteria
nonselective for Bacteroides spp.
nonselective for Clostridium spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)

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Thioglycollate medium

form

powder

form

powder

form

powder

form

powder

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

application(s)

clinical testing
food and beverages
sterility testing, microbiology

application(s)

agriculture
clinical testing
environmental
food and beverages
sterility testing, microbiology

application(s)

food and beverages
sterility testing, microbiology

application(s)

clinical testing
environmental
food and beverages
sterility testing

pH

7.2±0.2 (25 °C)

pH

-

pH

-

pH

-

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

manufacturer/tradename

NutriSelect® Plus

Application

Used for testing the sterility of biological products.

Components

Components (g/L)
Proteose Peptone 10.00
Infusion from Beef 17.50
Dextrose 5.00
Sodium Chloride 5.00
Dipotassium Phosphate 2.00
Sodium Thioglycollate 0.50
Methylene Blue 0.002
Agar 0.50

Preparation Note

Suspend 40.5 g of Brewer Thioglycollate Medium in 1000 ml of distilled water. Boil to dissolve the medium completely. Dispense in tubes or in suitable containers as desired and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes.Note: If more than the upper one third of medium has acquired a green color, the medium may be restored once by heating on a water bath or free flowing steam until the green color disappears.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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