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D2535

Sigma-Aldrich

Direct Blue 15

suitable for Histopaque® system, Powder

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About This Item

Linear Formula:
C34H24N6O16S4Na4
CAS Number:
Molecular Weight:
992.80
Colour Index Number:
24400
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Direct Blue 15, suitable for Histopaque® system, suitable for viability studies of collagenase-treated rat liver cells

description

suitable for Histopaque® system

Quality Level

form

powder

composition

Dye content, ~44%

color

dark blue

solubility

water: 10 mg/mL, clear, blue

suitability

suitable for viability studies of collagenase-treated rat liver cells

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].COc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(OC)c4

InChI

1S/C34H28N6O16S4.4Na/c1-55-25-9-15(3-5-23(25)37-39-31-27(59(49,50)51)11-17-7-19(57(43,44)45)13-21(35)29(17)33(31)41)16-4-6-24(26(10-16)56-2)38-40-32-28(60(52,53)54)12-18-8-20(58(46,47)48)14-22(36)30(18)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

OLSOUGWNONTDCK-UHFFFAOYSA-J

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Application

May be used as a substitute for trypan blue for some biological and staining applications.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yuzhong Zhan et al.
Journal of hazardous materials, 187(1-3), 348-354 (2011-01-29)
Copper hydroxide nitrate (Cu(2)(OH)(3)NO(3)) was synthesized solvothermally in anhydrous ethanol and characterized by XRD, FTIR, TG-DTA and SEM. The peroxide degradation of an azo dye (Direct Blue 15) on this material was evaluated by examining catalyst loading, initial pH, hydrogen
F Rafii et al.
Applied and environmental microbiology, 59(6), 1731-1734 (1993-06-01)
The purified azoreductase and nitroreductase of Clostridium perfringens, which have similar electrophoretic properties, both reacted in a Western blot (immunoblot) with a polyclonal antibody raised against the azoreductase. The activity of both enzymes was enhanced by flavin adenine dinucleotide and
A Kai et al.
International journal of biological macromolecules, 20(3), 221-231 (1997-06-01)
The difference of influence of a certain kind of direct dye on the structure of nascent microbial cellulose was examined, with Direct Red 28 have a biphenylenebis(azo) skeletal structure; Direct Blue 1 having two hydroxyl, two methoxy and two sulfonate
A Shan et al.
Journal of chromatographic science, 26(9), 439-442 (1988-09-01)
Two structurally similar azo dyes, Direct Blue No. 6 and Direct Blue No. 15, are analyzed by separate reversed-phase high-performance liquid chromatographic (HPLC) systems utilizing a dual-wavelength UV/visible detector at 254 and 546 nm. Each dye contains more than 35
Nurdan Kasikara Pazarlioglu et al.
Water environment research : a research publication of the Water Environment Federation, 82(7), 579-585 (2010-07-31)
To investigate biodegradability by Trametes versicolor, five structurally different direct azo-dyes--Direct Black 38, Direct Blue 15 (DB 15), Direct Orange 26, Direct Green 6, and Direct Yellow 12--were studied. The DB 15 was determined as the best biodegradable dye by

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