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H3136

Sigma-Aldrich

Hematoxylin

certified by the Biological Stain Commission

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Synonym(s):
Natural Black 1
Empirical Formula (Hill Notation):
C16H14O6 · xH2O
CAS Number:
Molecular Weight:
302.28 (anhydrous basis)
Colour Index Number:
75290
Beilstein/REAXYS Number:
91400
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

mp

200 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O

InChI

1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1

InChI key

WZUVPPKBWHMQCE-XJKSGUPXSA-N

Gene Information

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This Item
H9627199664O7505
Hematoxylin certified by the Biological Stain Commission

H3136

Hematoxylin

Hematoxylin

H9627

Hematoxylin

Sudan Black B certified by the Biological Stain Commission

199664

Sudan Black B

Orcein synthetic, certified by the Biological Stain Commission

O7505

Orcein

grade

certified by the Biological Stain Commission

grade

-

grade

certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

mp

200 °C (dec.) (lit.)

mp

200 °C (dec.) (lit.)

mp

120-124 °C (lit.)

mp

-

General description

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.

Application

Hematoxylin has been used:
  • to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples
  • as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent
  • to stain lung tissue for histological analysis

Suitability

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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