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M8010

Sigma-Aldrich

N,N′-Dimethyl-9,9′-biacridinium dinitrate

used as chemiluminescent reagent

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Synonym(s):
9,9′-Bis(N-methylacridinium nitrate), Lucigenin
Empirical Formula (Hill Notation):
C28H22N4O6
CAS Number:
Molecular Weight:
510.50
Beilstein/REAXYS Number:
3901768
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

form

powder

mp

245-250 °C

solubility

acetic acid: 10 mg/mL

fluorescence

λex 319 nm; λem 384 nm (pH 9.0)
λex 360 nm; λem 449 nm (Reaction product)
λex 368 nm; λem 505 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[O-][N+]([O-])=O.[O-][N+]([O-])=O.C[n+]1c2ccccc2c(-c3c4ccccc4[n+](C)c5ccccc35)c6ccccc16

InChI

1S/C28H22N2.2NO3/c1-29-23-15-7-3-11-19(23)27(20-12-4-8-16-24(20)29)28-21-13-5-9-17-25(21)30(2)26-18-10-6-14-22(26)28;2*2-1(3)4/h3-18H,1-2H3;;/q+2;2*-1

InChI key

KNJDBYZZKAZQNG-UHFFFAOYSA-N

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1 of 4

This Item
C1154219215C0875
Leucocrystal Violet

219215

Leucocrystal Violet

Calcein Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

C0875

Calcein

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

powder

form

powder

solubility

acetic acid: 10 mg/mL

solubility

water: 50 mg/mL, clear, dark yellow

solubility

-

solubility

NaOH: 50 mg/mL

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

Application

N,N′-Dimethyl-9,9′-biacridinium dinitrate has been used in chemiluminescence assay to measure reactive oxygen species production and superoxide production.
Reagent used for the generation of chemiluminescence in the presence of a reductant and oxygen; it is employed in numerous chemiluminescent assays. Lucigenin is specific for superoxide anion radical.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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