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P5631

Sigma-Aldrich

o-Cresolphthalein Complexone

powder

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Synonym(s):
o-Cresolphthalein-3′,3′′-bis-methyleneiminodiacetic acid, o-Cresolphthalexon, Metal phthalein, Phthalein purple, Xylenylphthalein-bisiminodiacetic acid
Empirical Formula (Hill Notation):
C32H32N2O12
CAS Number:
Molecular Weight:
636.60
Beilstein:
381994
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

color

white to off-white

mp

181-185 °C (dec.) (lit.)

solubility

methanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1cc(cc(CN(CC(O)=O)CC(O)=O)c1O)C2(OC(=O)c3ccccc23)c4cc(C)c(O)c(CN(CC(O)=O)CC(O)=O)c4

InChI

1S/C32H32N2O12/c1-17-7-21(9-19(29(17)43)11-33(13-25(35)36)14-26(37)38)32(24-6-4-3-5-23(24)31(45)46-32)22-8-18(2)30(44)20(10-22)12-34(15-27(39)40)16-28(41)42/h3-10,43-44H,11-16H2,1-2H3,(H,35,36)(H,37,38)(H,39,40)(H,41,42)

InChI key

IYZPEGVSBUNMBE-UHFFFAOYSA-N

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This Item
C85778114375B5880
o-Cresolphthalein indicator grade

Sigma-Aldrich

C85778

o-Cresolphthalein

Bromocresol Purple BioReagent, suitable for indicator, Dye content 90 %

Sigma-Aldrich

114375

Bromocresol Purple

Bromocresol Purple Technical grade

Sigma-Aldrich

B5880

Bromocresol Purple

color

white to off-white

color

-

color

-

color

-

mp

181-185 °C (dec.) (lit.)

mp

219-221 °C (lit.)

mp

240 °C (dec.) (lit.)

mp

240 °C (dec.) (lit.)

solubility

methanol: 1 mg/mL

solubility

-

solubility

ethanol: 1 mg/mL

solubility

methanol: 4 mg/mL, orange

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

o-Cresolphthalein Complexone is a pH indicator dye commonly used for the calorimetric determination of calcium in serum.

Application

o-Cresolphthalein Complexone has been used in the estimation of calcium in serum samples based on the reaction of Ca2+ with o-cresolphthalein complexone in an alkaline solution.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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B Aghabeigi et al.
International journal of oral and maxillofacial surgery, 31(6), 646-649 (2003-01-11)
Saline aspirates have been commonly used in the biochemical investigations of temporomandibular joint (TMJ) pathology. However, due to presence of adhesions in the diseased temporomandibular joint, full equilibration between the injected saline and the synovial fluid may not be achieved
D H Patterson et al.
Journal of chromatography. A, 662(2), 389-395 (1994-02-25)
Electrophoretically mediated microanalysis (EMMA) was used for the determination of calcium. The faster analyte zone containing the calcium was injected spatially behind a slower zone of o-cresolphthalein complexone in a capillary electrophoresis based system. Upon application of the electric field
C M Corns
Annals of clinical biochemistry, 27 ( Pt 2), 152-155 (1990-03-01)
The spectral interference caused by haemoglobin in the calcium-cresolphthalein complexone reaction was investigated. The results show that the nature of the haemoglobin derivative in the reaction mixture influences the degree of interference; in turn, the derivative formed depends on the
James H Wible et al.
Radiology, 233(1), 158-164 (2004-09-30)
To measure serum calcium concentration with three different analytic methods after administration of gadoversetamide and three other gadolinium chelates in dogs. Six dogs were injected with 0.1-, 0.3-, 1.0-, and 3.0-mmol/kg doses of gadoversetamide; 1.0-mmol/kg doses of gadodiamide, gadopentetate, and
C M Corns et al.
Annals of clinical biochemistry, 24 ( Pt 4), 345-351 (1987-07-01)
The reasons for the non-linearity of the standard curve for the calcium-cresolphthalein complexone reaction have been investigated. Calcium forms both 1:1 and 2:1 complexes with cresolphthalein complexone; at low calcium concentrations the 1:1 complex predominates and causes non-linearity. At high

Protocols

Suitability for the qualitative determination of calcium with phthalein purple (O-cresophthalein complexone) using stannous chloride

Suitability for the Qualitative Determination of Calcium with Phthalein Purple (O-Cresophthalein Complexone) using Stannous Chloride

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