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RDD028

Sigma-Aldrich

Ethanolamine hydrochloride

anhydrous, free-flowing, Redi-Dri, ≥99.0%

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Synonym(s):
Colamine hydrochloride, Ethanolamine chloride, 2-Aminoethanol hydrochloride
Linear Formula:
H2NCH2CH2OH · HCl
CAS Number:
Molecular Weight:
97.54
Beilstein/REAXYS Number:
3542892
MDL number:

grade

anhydrous

Quality Level

product line

Redi-Dri

assay

≥99.0%

quality

free-flowing

pKa (25 °C)

9.5

mp

82-84 °C (lit.)

SMILES string

NCCO.[H]Cl

InChI

1S/C2H7NO.ClH/c3-1-2-4;/h4H,1-3H2;1H

InChI key

PMUNIMVZCACZBB-UHFFFAOYSA-N

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General description

Ethanolamine hydrochloride, a versatile compound in biochemistry and molecular biology, plays a pivotal role in diverse research areas. Primarily, it functions as a buffering agent, ensuring a stable pH environment crucial for enzymatic reactions, protein solubilization, and other biological processes. Its significance extends to membrane protein studies, aiding in the extraction of these proteins from cell membranes. It also finds utility in cell culture and as a reagent for biological sample preparation, simplifying nucleic acid and protein isolation, as well as facilitating the detection of proteins by western blotting and immunohistochemistry. This compound′s wide-ranging applications make it indispensable in biochemical research, contributing to various experimental procedures and advancing our understanding in fields such as molecular biology, immunology, and cellular biology.

Application

Redi-Dri is an innovative packaging product line that helps eliminate clumping material by adding extra packaging process steps without any chemical additives, anti-clumping agents, or hydrophobic compounds. Redi-Dri offers a more convenient and safer way to handle the products.
Ethanolamine hydrochloride has been used:
  • in overnight incubation of the tips to attach primary amine groups at the tip surface
  • in the preparation of DMEM (dulbecco′s modified eagle′s medium)/F-12 media to culture human epidermal growth factor receptor 2 (HER2) cells derived from MMTV-HER2 transgenic mouse mammary tumors
  • to administer the cultures to study its effect on the endogenous phosphatidyl ethanolamine pool and autophagy process

Biochem/physiol Actions

Ethanolamine hydrochloride is utilized as a carbon and nitrogen source by bacteria that differs phylogenetically. It exists as phosphotidylethanolamine in the bacterial and mammalian cell membrane. Ethanolamine-ammonia lyase is responsible for the degradation of ethanolamine into acetaldehyde and ammonia. Ethanolamine is known to positively support lipid accumulation in photosynthetic organism model.

Features and Benefits

  • Suitable for molecular biology
  • Redi-Dri packaging controls moisture for hygroscopic chemicals that commonly clump
  • Powder remains free-flowing and easy to use
  • Safer to handle and weigh out for routine use in the lab
  • Eliminates clumps, maintaining product moisture, quality, and product specifications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Redi-Dri™ prevents hygroscopic powders, such as inorganic salts, from absorbing moisture and forming clumps, leaving the salts free-flowing every time.

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