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S7907

Sigma-Aldrich

Sodium phosphate dibasic

BioXtra, ≥99.0%

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Synonym(s):
sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate
Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
EC Number:
MDL number:
eCl@ss:
38070211
PubChem Substance ID:
NACRES:
NA.25

product line

BioXtra

Quality Level

Assay

≥99.0%

form

powder or crystals

impurities

≤0.1% Insoluble matter

pH

8.0-11.0 (20 °C, 1 M in H2O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.002%
Cu: ≤0.0005%
Fe: ≤0.002%
K: ≤0.005%
Mg: ≤0.005%
NH4+: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

<0.05 at 260 in H2O at 1 M
<0.05 at 280 in H2O at 1 M

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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Sodium phosphate dibasic BioXtra, &#8805;99.0%

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S7907

Sodium phosphate dibasic

Premium Grade
Sodium phosphate dibasic BioUltra, for molecular biology, &#8805;99.5% (T)

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71636

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Sodium phosphate dibasic ReagentPlus&#174;, &#8805;99.0%

Sigma-Aldrich

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Sodium phosphate dibasic BioReagent, suitable for cell culture, suitable for insect cell culture, &#8805;99.0%

Sigma-Aldrich

S5136

Sodium phosphate dibasic

Essential+ Grade
assay

≥99.0%

assay

≥99.5% (T)

assay

≥99.0%

assay

≥99.0%

form

powder or crystals

form

coarse powder, fine crystals

form

powder or crystals

form

powder or crystals

impurities

≤0.1% Insoluble matter

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% total nitrogen (N)

impurities

-

impurities

-

pH

8.0-11.0 (20 °C, 1 M in H2O)

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

pH

8.9-9.2 (25 °C, 50 g/L in water)

pH

8.9-9.2 (25 °C, 50 g/L in water)

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

water: soluble 100 mg/mL

solubility

water: soluble 100 mg/mL

General description

Sodium phosphate dibasic (NaH2PO4), also known as disodium phosphate, is a commonly used phosphate salt in biochemistry research due to its versatile properties. It is a key component in the preparation of phosphate buffer saline (PBS) and phosphate buffer, a commonly used buffer solution in many biological applications. In addition to its buffer properties, NaH2PO4 is also used as a precipitating agent to form silver orthophosphate (Ag3PO4) powders, making it a valuable tool in many laboratory settings. Disodium phosphate is also a widely used emulsifier in dairy products such as cheese and other foods.

Application

Sodium phosphate dibasic has been used:
  • as a component of phosphate buffered saline (PBS)
  • as a component of buffer for the preparation of cryopreservation diluent
  • in the preparation of McIlvaine buffer (citrate-phosphate buffer) for toluidine blue staining
  • in the preparation of the S9 mix for the Ames test, and SOS Chromotest
  • in the preparation of calcium deficient hydroxyapatite (CDHA) paste during the fabrication process of the CDHA scaffold

Features and Benefits

  • The BioXtra product line ensures high-quality products for consistent results.
  • High level of assay ( ≥99.0%), indicating its purity.
  • Powder form for easy handling and use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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