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S3264

Sigma-Aldrich

Sodium phosphate dibasic

for molecular biology, ≥98.5% (titration)

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Synonym(s):
sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate
Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
EC Number:
MDL number:
eCl@ss:
38070211
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

Assay

≥98.5% (titration)

form

powder

pH

8.9-9.2 (25 °C, 50 g/L in water)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: 100 mg/mL

anion traces

chloride (Cl-): ≤40 ppm

cation traces

Fe: ≤20 ppm
heavy metals (as Pb): ≤10 ppm

application(s)

diagnostic assay manufacturing

foreign activity

DNase, RNase and protease, none detected

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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Sodium phosphate dibasic for molecular biology, ≥98.5% (titration)

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Sodium phosphate dibasic purum p.a., anhydrous, ≥98.0% (T)

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71642

Sodium phosphate dibasic

-
assay

≥98.5% (titration)

assay

≥99.0%

assay

≥99.5% (T)

assay

≥98.0% (T)

form

powder

form

powder or crystals

form

coarse powder, fine crystals

form

powder

pH

8.9-9.2 (25 °C, 50 g/L in water)

pH

8.9-9.2 (25 °C, 50 g/L in water)

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

pH

8.8-9.4 (25 °C, 50 mg/mL in H2O)

solubility

H2O: 100 mg/mL

solubility

water: soluble 100 mg/mL

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

water: soluble 50 mg/mL at 25 °C

anion traces

chloride (Cl-): ≤40 ppm

anion traces

-

anion traces

chloride (Cl-): ≤10 mg/kg, sulfate (SO42-): ≤50 mg/kg

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤1000 mg/kg

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.

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