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71636

Sigma-Aldrich

Sodium phosphate dibasic

BioUltra, for molecular biology, ≥99.5% (T)

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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About This Item

Linear Formula:
Na2HPO4
CAS Number:
7558-79-4
Molecular Weight:
141.96
EC Number:
231-448-7
MDL number:
MFCD00003496
UNSPSC Code:
12161700
eCl@ss:
38070211
PubChem Substance ID:
57652328
NACRES:
NA.25

grade

for molecular biology

product line

BioUltra

assay

≥99.5% (T)

form

coarse powder
fine crystals
powder

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.001% total nitrogen (N)

loss

≤0.1% loss on drying, 110 °C

pH

8.8-9.4 (25 °C, 0.5 M in H2O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.2 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤10 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.025
λ: 280 nm Amax: ≤0.020

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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Application

Sodium phosphate dibasic has been used:
  • as a component in phosphate buffer
  • in the preparation of MgCl2 hybridization buffer
  • as a component in SSPE solution for the preparation of small RNA hybridisation buffer

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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