SFRVN

Sf-RVN^™ Insect Cell Line

• Synonym(s): Sf9 rhabdovirus-negative (Sf-RVN^™) Insect Cell Line

Properties

• description: Sf9 rhabdovirus-negative (Sf-RVN^™) Insect Cell Line
• solubility: DMSO: 10%
• shipped in: liquid nitrogen
• storage temp.: −196°C

Description

General description

Sf-RVN(R)cells are provided to customers in vials containing 1 mL at 10 x 106 cells/mL. Cells are banked in EX-CELL^® CD Insect Cell Medium and 10% DMSO.

The Sf-RVN^™ Insect Cell Line is a rhabdovirus-negative Sf9 cell line that can be grown in both adherent and suspension culture. Sf-RVN^™ Insect Cell Line has been adapted for growth in chemically defined medium to be used for the production of recombinant proteins, adeno-associated viruses (AAV) and viral-like particles (VLP). The cell line was tested for adventitious agents and banked under GMP conditions. A technical user guide, with detailed protocols for optimal performances, is provided with the cell line.The EX-CELL^® CD Insect Cell Medium, recommended for use, is optimized to get excellent growth and productivity of the Sf-RVN^™ Insect Cell Line. Combined, these two products form the Sf-RVN^™ Platform.

Cell Line Origin

Sf-RVN^™ is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith and C. Cherry. Sf-RVN^™ was isolated from Sf9 by GlycoBac, LLC.

Physical form

Sf-RVN^™ cells are provided to customers in vials containing 1 mL at 10 x 10⁶cells/mL. Cells are banked in EX-CELL^® CD Insect Cell Medium and 10% DMSO

Other Notes

Sf-RVN^™ is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith and C. Cherry. Sf-RVN ^™ was isolated from Sf9 by GlycoBac, LLC.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

EXACT-AIR is a registered trademark of Merck KGaA, Darmstadt, Germany

Sf-RVN is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable