MilliporeSigma
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14390C

SAFC

Cellvento® 4CHO-C Cloning Medium

Animal- component free, chemically defined

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sterility

sterile; sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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C636614385C24340C
Cellvento® 4CHO-C Cloning Medium Animal- component free, chemically defined

SAFC

14390C

Cellvento® 4CHO-C Cloning Medium

SAFC

SAFC

C6366

EX-CELL® CHO Cloning Medium

EX-CELL® CD HEK293 Viral Vector Medium Chemically defined, animal component-free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

SAFC

14385C

EX-CELL® CD HEK293 Viral Vector Medium

SAFC

SAFC

24340C

EX-CELL® 325 PF CHO Serum-Free Medium for CHO Cells

sterility

sterile; sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

form

liquid

form

liquid

form

liquid

form

dry powder

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no

components

L-glutamine: no
NaHCO3: yes

components

L-glutamine: no

components

-

shipped in

ambient

shipped in

ambient

shipped in

wet ice

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Cellvento® 4CHO-C Cloning Medium supports CHOZN® and CHO cell line development and has been developed to replace hydrolysate containing EX-CELL® CHO Cloning Medium (C6366) at comparable performance. It provides chemically defined media solutions for the entire process from cell line development to production scale.
Chemically definded medium

Application

Cell Culture, Single Cell Cloning
Developed to meet the needs of the biotechnolgy industry, this medium is designed for single cell cloning, stable pool selection and recovery of Chinese Hamster Ovary (CHO) cell lines adapted to suspension culture.

Features and Benefits

Developed to meet the needs of the biotechnology industry, this medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free and hydrolysate free suspension culture.

Packaging

Pack sizes: 14390C-500ML

Components

L-glutamine: no

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lenthly periods of time.

Reconstitution

Aspectically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use (if applicable).

Other Notes

The formulation is designed without hydrolysates or components of unknown composition. It contains hypoxanthine and thymidine (HT) but for more selcection flexibility, it does not contain L-glutamine, Methionine sulfoximine (MSC), or Methotrexate (MTX).

Legal Information

6538 is a trademark of American Type Culture Collection
CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany
CHOZN is a registered trademark of Sigma-Aldrich Co. LLC
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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