MilliporeSigma
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C6366

SAFC

EX-CELL® CHO Cloning Medium

Animal-component free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | mammalian: suitable (suspension)

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

form

liquid

form

liquid

form

liquid

form

dry powder

storage condition

protect from light

storage condition

-

storage condition

-

storage condition

-

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

-

components

L-glutamine: no
NaHCO3: yes

components

phenol red: yes
L-glutamine: no
NaHCO3: 1.6 g/L
glucose: 5.6 g/L
HEPES: 7.5 mM

components

L-glutamine: no

components

-

Application

This animal component-free medium is designed to support clonal survival and growth of Chinese Hamster Ovary (CHO) cell lines, with results comparable to the traditional method using 10% fetal bovine serum.

Features and Benefits

Developed to meet the needs of the biotechnology industry, this animal component-free medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free suspension culture.

Other Notes

Proprietary formulation containing inorganic salts, sodium bicarbonate, essential and non-essential amino acids, vitamins, trace elements, plant hydrolysates, and other organic compounds.
Does not contain antibiotics, antimycotics, L-glutamine, insulin, or transferrin.

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Reconstitution

Aseptically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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