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SMB00937

Sigma-Aldrich

Fatty Acid Methyl Esters Standard Mixture

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Synonym(s):
FAME, fatty acids, standard mixture

form

liquid

Quality Level

packaging

pkg of 1 vial

solubility

soluble, clear, colorless

storage temp.

-10 to -25°C

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This Item
49454-UFAMLS47080-U
packaging

pkg of 1 vial

packaging

ampule of 1 mL

packaging

pkg of 1 plates

packaging

ampule of 1 mL

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

solubility

soluble, clear, colorless

solubility

-

solubility

-

solubility

-

storage temp.

-10 to -25°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-10 to -25°C

General description

The Fatty Acid Methyl Esters Standard Mixture is a metabolite mixture comprised of 13 fatty acids. Fatty acid methyl esters are dissolved in chloroform to the stated concentrations of ± 4%. This product was formulated for use as a retention marker for high-throughput GC/MS metabolomic analysis.

Application

Used as a QC sample for GC-MS-based metabolomics studies.

Gas chromatography-mass spectrometry (GC-MS)-based metabolomics is used to identify and quantify small-molecule metabolites such as small acids, alcohols, hydroxyl acids, amino acids, sugars, fatty acids, sterols, catecholamines, drugs, and toxins.

Fatty acid methyl esters used in this standard showed a strong, consistent m/z 87 ion and molecular ions which could be used to differentiate the individual peaks.

Analyte

Description

    Methyl octanoate 800 µg/mL
    Methyl nonanoate 800 µg/mL
    Methyl decanoate 800 µg/mL
    Methyl laurate 800 µg/mL
    Methyl myristate 800 µg/mL
    Methyl palmitate 800 µg/mL
    Methyl stearate 400 µg/mL
    Methyl arachidate 400 µg/mL
    Methyl behenate 400 µg/mL
    Methyl tetracosanoate 400 µg/mL
    Methyl hexacosanoate 400 µg/mL
    Methyl octacosanoate 400 µg/mL
    Methyl triacontanoate 400 µg/mL
    See All (13)

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Salvatore Abate et al.
Rapid communications in mass spectrometry : RCM, 24(8), 1172-1180 (2010-03-20)
Many metabolomic applications use gas chromatography/mass spectrometry (GC/MS) under standard 70 eV electron ionization (EI) parameters. However, the abundance of molecular ions is often extremely low, impeding the calculation of elemental compositions for the identification of unknown compounds. On changing
Tobias Kind et al.
Analytical chemistry, 81(24), 10038-10048 (2009-11-26)
At least two independent parameters are necessary for compound identification in metabolomics. We have compiled 2 212 electron impact mass spectra and retention indices for quadrupole and time-of-flight gas chromatography/mass spectrometry (GC/MS) for over 1000 primary metabolites below 550 Da
Oliver Fiehn
Current protocols in molecular biology, 114, 30-30 (2016-04-03)
Gas chromatography-mass spectrometry (GC-MS)-based metabolomics is ideal for identifying and quantitating small-molecule metabolites (<650 Da), including small acids, alcohols, hydroxyl acids, amino acids, sugars, fatty acids, sterols, catecholamines, drugs, and toxins, often using chemical derivatization to make these compounds sufficiently

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