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T0138

Sigma-Aldrich

Tetrazolium Violet

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Synonym(s):
2,5-Diphenyl-3-(α-naphthyl)tetrazolium chloride, 2,5-Diphenyl-3-(1-naphthyl)tetrazolium chloride, TV
Empirical Formula (Hill Notation):
C23H17ClN4
CAS Number:
Molecular Weight:
384.86
EC Number:
MDL number:
PubChem Substance ID:

assay

≥98% (TLC)

Quality Level

form

powder

mp

247-249 °C

solubility

methanol: 1 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4cccc5ccccc45

InChI

1S/C23H17N4.ClH/c1-3-11-19(12-4-1)23-24-26(20-14-5-2-6-15-20)27(25-23)22-17-9-13-18-10-7-8-16-21(18)22;/h1-17H;1H/q+1;/p-1

Inchi Key

RONADMZTCCPLEF-UHFFFAOYSA-M

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1 of 4

This Item
M21288819061135
vibrant-m

T0138

Tetrazolium Violet

vibrant-m

M2128

Thiazolyl Blue Tetrazolium Bromide

vibrant-m

88190

Tetrazolium Blue Chloride

vibrant-m

61135

Crystal Violet

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

assay

≥98% (TLC)

assay

98%

assay

≥90% (T)

assay

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

microbiology

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

methanol: 1 mg/mL, clear

solubility

2-methoxyethanol: soluble 20 mg/mL, H2O: 5 mg/mL

solubility

methanol: 0.5 g/10 mL ( hot)

solubility

-

General description

Tetrazolium Violet is a monotetrazolium salt which forms water-insoluble formazans. These salts are mainly used as indicators of extracellular redox activity and cell redox potential. Tetrazolium violet is positively charged and cell-permeable.

application

Tetrazolium Violet has been used to stain agar layers in bacteriophage plaque formation assays.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Experimental conditions affect the site of tetrazolium violet reduction in the electron transport chain of Lactococcus lactis
Sybille Tachon
Microbiology, 2941-2948 (2009)
Differential Effect of Tetrazolium Dyes upon Bacteriophage Plaque Assay Titers
CHRISTON J. HURST
Applied and Environmental Microbiology, 3462-3465 (1994)
J Gianoudis et al.
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