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10065

Sigma-Aldrich

α-Amylase from Aspergillus oryzae

powder, ~30 U/mg

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Synonym(s):
1,4-α-D-Glucan-glucanohydrolase
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.54

form

powder

Quality Level

specific activity

~30 U/mg

mol wt

Mr ~51000

storage temp.

2-8°C

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This Item
A985786247A8220
vibrant-m

10065

α-Amylase from Aspergillus oryzae

vibrant-m

A9857

α-Amylase from Aspergillus oryzae

vibrant-m

A8220

α-Amylase from Aspergillus oryzae

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

mol wt

Mr ~51000

mol wt

-

mol wt

-

mol wt

-

form

powder

form

powder

form

powder

form

aqueous solution

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Microbial amylases are exoenzymes and are used in several industrial applications, such as production of bread, maltose syrups, and fermentation of soya sauce, miso etc. α-Amylase isolated from Aspergillus oryzae has been found to have molecular weights of 51kDa (sedimentation and diffusion) and 49kDa (gel filtration).

Application

α-Amylase has been used:
  • as a control enzyme in agar plate-based and carboxymethylcellulose-based clearing assays to screen cellulase activity
  • for the hydrolysis of starch to explore the the role of wheat starch in frozen dough
  • to inhibit Staphylococcus aureus biofilms

Biochem/physiol Actions

Aspergillus oryzae α-amylase (Ao α-amylase) enzyme catalyzes the hydrolysis of the α-1,4 glycosidic bonds in soluble starches and related subsrates. These substrates are broken down to release short oligosaccharides and α-limit dextrins.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.0 and 25°C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).

Other Notes

For the determination of fats in food; Application in (selective) hydrolysis/condensation of glycosidic bonds.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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