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Sigma-Aldrich

Anti-Rabbit IgG - Atto 488 antibody produced in goat

whole molecule, 1 mg/mL protein

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Synonym(s):
Atto 488-[goat-Anti-rabbit IgG], whole molecule
NACRES:
NA.46

conjugate

Atto 488 conjugate

Quality Level

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

contains

50% glycerol

species reactivity

rabbit

concentration

1 mg/mL protein

technique(s)

immunocytochemistry: 1:500
immunofluorescence: 1:200
immunohistochemistry: suitable

fluorescence

λex 485 nm; λem 552 nm in PBS

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
A3687A542036098
species reactivity

rabbit

species reactivity

rabbit

species reactivity

goat

species reactivity

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

technique(s)

immunocytochemistry: 1:500, immunohistochemistry: suitable, immunofluorescence: 1:200

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:40,000, dot blot: 1:80,000-1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

technique(s)

-

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

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General description

Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody antibody reats with rabbit IgG in vitro, in rabbit serum and biological fluids.
Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Atto 488 is a labeling dye with high molecular absorption (90,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. It is optimized for excitation with an argon laser, and is characterized by high photostability. Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Immunogen

Rabbit IgG

Application

Anti-Rabbit IgG has been used for immunocytochemistry applications at a dilution of 1:500 in neural precursor cells.
Goat polyclonal anti-Rabbit IgG (whole molecule)-Atto 488 antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids via fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use rabbit IgG as the primary antibody. Atto 488 goat anti-rabbit IgG antibody was used for immunofluorescence to detect L. monocytogenes in mouse tissue sections following incubation with an anti-listeria antibody. Antibody was used at a 1:200 dilution.

Physical form

Atto 488 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Analysis Note

Unconjugated dye ≤5% of total fluorescence; dye-to-protein ratio >2.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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