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SAB4600234

Sigma-Aldrich

Anti-Rabbit IgG (H+L), CF488A antibody produced in F(ab')2 fragment of goat

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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NACRES:
NA.46

biological source

goat

Quality Level

conjugate

CF 488A conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

concentration

~2 mg/mL

technique(s)

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1-10 μg/mL
western blot: suitable

fluorescence

λex 490 nm; λem 515 nm

storage temp.

−20°C

target post-translational modification

unmodified

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SAB4600030SAB4600310SAB4600362
biological source

goat

biological source

chicken

biological source

goat

biological source

goat

technique(s)

flow cytometry: 1-10 μg/mL, immunohistochemistry: suitable, indirect immunofluorescence: 1-10 μg/mL, immunocytochemistry: suitable, western blot: suitable, indirect ELISA: suitable

technique(s)

flow cytometry: 1-10 μg/mL, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect immunofluorescence: 1-10 μg/mL

technique(s)

flow cytometry: 1-10 μg/mL, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, indirect immunofluorescence: 1-10 μg/mL, western blot: suitable

technique(s)

flow cytometry: 1-10 μg/mL, immunocytochemistry: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, indirect immunofluorescence: 1-10 μg/mL, western blot: suitable

conjugate

CF 488A conjugate

conjugate

CF 488A conjugate

conjugate

CF 568 conjugate

conjugate

CF 680 conjugate

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
This product is prepared by labeling high quality F(ab′)2 fragment of goat anti-rabbit IgG (H+L) with the green fluorescent dye CF488A. CF488A is a superior alternative to the traditional green fluorescent dye FITC (or fluorescein) owing to its exceptional brightness and photostability. CF488A is comparably bright and photostable to Alexa Fluor 488. However, antibody conjugates prepared from CF488A are more specific than those prepared from Alexa Fluor 488 because CF488A, being minimally charged, does not significantly change the isoelectric points of antibodies as Alexa Fluor 488 does

Specificity

Binds with whole molecule rabbit IgG and the light chains of other rabbit immunoglobulins.

Immunogen

rabbit IgG (H+L)

Application

Anti-Rabbit IgG (H+L), CF 488A antibody has been used in western blotting and immunostaining.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Preparation Note

Protect from light. The antibody solution should be gently mixed before use.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Aihua Hou et al.
Eye and vision (London, England), 4, 17-17 (2017-07-15)
Gram-positive bacteria stimulate Toll-like receptor (TLR) 2 and then activate the pro-inflammatory nuclear factor-kappa B (NF-κB) pathway. As the human ocular surface is heavily colonised by gram-positive cocci bacteria, a balance of activation/repression of NF-κB target genes is essential to
Plastic embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging.
Gang Y, et al.
Biomedical Optics Express, 8(8), 3583-3596 (2017)
Toll-like receptor 2-mediated NF-kappa B pathway activation in ocular surface epithelial cells.
Hou A, et al.
Eye and Vision (London, England), 4(1), 17-17 (2017)
Johannes Walter et al.
International journal of molecular sciences, 24(16) (2023-08-26)
Activation of the interleukin-4 (IL-4) pathway ameliorates secondary injury mechanisms after experimental traumatic brain injury (TBI); therefore, we assessed the effect of a therapeutic IL-4 administration on secondary brain damage after experimental TBI. We subjected 100 C57/Bl6 wildtype mice to
Metformin ameliorates sepsis-induced brain injury by inhibiting apoptosis, oxidative stress and neuroinflammation via the PI3K/Akt signaling pathway.
Tang G, et al.
Oncotarget, 8(58), 97977-97977 (2017)

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