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Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Atto 488 antibody produced in goat

~1 mg/mL

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NACRES:
NA.46

biological source

goat

Quality Level

conjugate

Atto 488 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

50% glycerol as stabilizer

species reactivity

mouse

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 1:500
immunofluorescence: 1:400

fluorescence

λex 500 nm; λem 522 nm in PBS

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
A3438A1047A3563
biological source

goat

biological source

goat

biological source

goat

biological source

sheep

conjugate

Atto 488 conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

form

liquid

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

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General description

IgG is the most abundant antibody in human serum. It is secreted by B cells andis found in blood. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Anti-Mouse IgG (whole molecule)-Atto 488 antibody is specific for mouse IgG. The product is purified from goat anti-mouse IgG antiserum to remove all goat serum proteins, including immunoglobulins that do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity.

Immunogen

purified mouse IgG.

Application

Anti-Mouse IgG (whole molecule)?Atto 488 antibody produced in goat has been used:
  • in immunofluorescence confocal microscopic assay for p65 localization
  • in immunohistochemical analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

IgG facilitates transplacental transport and participates in the secondary immune response. It has the longest serum half life. It might be linked to allergy. IgG initiates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC).

Physical form

Solution in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, andcontaining 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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IgG subclasses and allotypes: from structure to effector functions
Vidarsson G, et al.
Frontiers in Immunology, 5(1-2), 520-520 (2014)
Arnold Garbiec et al.
Arthropod structure & development, 41(2), 165-176 (2012-02-04)
Mechanisms that underlie differentiation and diversification of the ovarian follicular epithelium in insects have been best characterized in a fruit fly, Drosophila melanogaster. Recent comparative analyses have shown that dipterans evolved a common, specific system of early patterning of their
Emily J Reedich et al.
Journal of neuroscience research, 100(10), 1951-1966 (2022-07-16)
Spastic cerebral palsy (CP) is a movement disorder marked by hypertonia and hyperreflexia; the most prevalent comorbidity is pain. Since spinal nociceptive afferents contribute to both the sensation of painful stimuli as well as reflex circuits involved in movement, we
Carlo Sala Frigerio et al.
Cell reports, 27(4), 1293-1306 (2019-04-25)
Gene expression profiles of more than 10,000 individual microglial cells isolated from cortex and hippocampus of male and female AppNL-G-F mice over time demonstrate that progressive amyloid-β accumulation accelerates two main activated microglia states that are also present during normal
Jan Andreas Ruland et al.
Nature communications, 12(1), 6211-6211 (2021-10-29)
Ribosomal biogenesis has been studied by biochemical, genetic and electron microscopic approaches, but live cell data on the in vivo kinetics are still missing. Here we analyse the export kinetics of the large ribosomal subunit (pre-60S particle) through single NPCs

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