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22178

Sigma-Aldrich

Cellulase from Aspergillus niger

powder, off-white, ~0.8 U/mg

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Synonym(s):
1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

Aspergillus niger

form

powder

specific activity

~0.8 U/mg

color

off-white

storage temp.

2-8°C

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This Item
C8546C1184A2910
form

powder

form

lyophilized powder

form

powder

form

powder

specific activity

~0.8 U/mg

specific activity

≥1 unit/mg solid

specific activity

≥0.3 units/mg solid

specific activity

-

color

off-white

color

-

color

-

color

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.

Application

Cellulase from Aspergillus niger has been used:
  • in the preparation of the standardized solution to determine absolute cellulase levels
  • in phosphate-citric acid buffer for activity assay
  • in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed

Biochem/physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.

Unit Definition

1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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