All Photos(2)




Human Fibroblast-Like Synoviocytes-Osteoarthritis: HFLS-OA, adult

Sign Into View Organizational & Contract Pricing


biological source

human synovial tissues (osteoarthritic)

Quality Level


pkg of 500,000 cells


Cell Applications, Inc

growth mode



2n = 46




cell culture | mammalian: suitable

relevant disease(s)

arthritis; coagulation disorders

shipped in

dry ice

storage temp.


Related Categories

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

HFLS-RA provide an excellent cellular model for studying synoviocyte physiology in relation to development and treatment of rheumatoid arthritis.

HFLS-RA have been used in numerous research studies to:
  • Study signaling pathways implicated in the development of joint inflammation and rheumatoid arthritis (Abe, 2006; Akasaka, 2005; Bai, 2012; Fan, 2013; Furuhashi, 2008; Hashizume, 2008; Matsuyama, 2010; Tsuji, 1999; Yamaguch, 2012a,b; Yano, 2001)
  • Evaluate possible contribution of EBV to nonresolving rheumatoid arthritis inflammation through inducing IL-6 production by synoviocytes (Chiu, 2013)
  • Evaluate the anti-inflammatory and antirheumatic activity of various compounds, such as celastrol (Venkatesha, 2011; Xu, 2013), triptolide (Kong, 2013; Lin, 2013), arsenic trioxide (Mei, 2011), Wen Luo Yin (Liu, 2013), bucillamine (Tsuji, 2007), therapeutic lyposomes (Harigai, 2007; Ichihara, 2011; Matsumoto, 2013; Negishi, 2011), anti-IL-6R and anti-CD319 antibodies (Suzuki, 2010; Woo, 2013), as well as inhibitors of NF-κB (Baima, 2010; Keith, 2005), methionine aminopeptidase-2 (Bernier, 2004), Tpl2 (Hall, 2007), aryl hydrocarbon receptor (Lahoti, 2013) and p38 MAP kinase (Wada, 2005); and additionally demonstrate the beneficial anti-inflammatory effects of low level light therapy (Yamaura, 2009)
  • Investigate the causes of metalloproteinases induction in patients with Lyme disease-associated arthritis (Lin, 2001)
  • Develop adenovirus-based siRNA delivery system (Arts, 2003)

Additionally, together with normal HFLS, HFLS-RA were used to:
  • Identify causing agents (such as uric acid crystals or platelet microparticles) and study the immunopathological mechanisms and signal transduction pathways leading to joint inflammation in rheumatoid arthritis (Chen, 2011a; Hsu, 2012; Mathieu, 2008; Tsuji, 2012), and to demonstrate the role of estrogen signaling in increasing inflammation (Galal, 2008)
  • Investigate anti-inflammatory properties of herbal compound Sinomenine suggested for rheumatoid arthritis treatment (Chen, 2011b)
  • Study the effects of extracellular matrix composition on cell attachment and migration relevant to T-cell function in inflamed tissues (Evanko, 2012)

In parallel with HFLS isolated from joints of patients with osteoarthritis HFLS-RA were used in a study demonstrating the involvement of capsid proteins of parvorvirus B19 in activating synoviocyte migration and induction of the inflammatory response leading to acute symmetrical polyarthropathy (Lu, 2006).

Finally, all three types of HFLS (normal, RA and OA) were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms (Freimanis, 2010).

Cell Line Origin



synoviocyte physiology in relation to development and treatment of rheumatoid arthritis, phenotype, proinflammatory mediator production, signaling pathways, interleukin production, evaluation of anti-inflammatory and antirheumatic compounds, erosive enzyme production (matrix metalloproteinases), cell proliferation and attraction


Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 5 doublings

Subculture Routine

Please refer to the HFLS-OA Culture Protocol.


RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

11 - Combustible Solids




Not applicable


Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service