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Sigma-Aldrich

Anti-Rabbit-IgG - Atto 550 antibody produced in goat

Synonym(s):

Atto 550-Anti-Rabbit-IgG antibody produced in goat

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About This Item

MDL number:
MFCD06798560
UNSPSC Code:
12352203
NACRES:
NA.46

conjugate

Atto 550 conjugate

Quality Level

100

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

50% glycerol as stabilizer

species reactivity

rabbit

concentration

1 mg/mL protein

technique(s)

immunofluorescence: suitable

fluorescence

λex 550 nm; λem 576 nm in PBS

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs are known to associate with rabbit IgGs.

Immunogen

rabbit IgG

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 550-goat anti-rabbit-IgG has been used for immunofluorescence applications.

Physical form

Atto 550 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Maximilian G Burger et al.
International journal of molecular sciences, 18(12) (2017-12-01)
The accumulation of senescent cells is implicated in the pathology of several age-related diseases. While the clearance of senescent cells has been suggested as a therapeutic target for patients with osteoarthritis (OA), cellular senescence of bone-resident osteoblasts (OB) remains poorly
Unraveling astrocyte behavior in the space brain: Radiation response of primary astrocytes.
Roggan, et al.
Frontiers in public health, 11, 1063250-1063250 (2023)
Orhan Tansel Korkmaz et al.
Journal of molecular neuroscience : MN, 41(2), 278-287 (2009-12-03)
The ventral anterior nucleus of the thalamus (VATh) gathers motor information from the internal segment of the globus pallidus (GPi) and substantia nigra pars reticulata (SNpr) of the basal ganglia and projects directly to motor areas of cortex. GPi/SNpr send
Máté Sági-Kazár et al.
Frontiers in plant science, 12, 658987-658987 (2021-06-08)
Iron (Fe) is an essential micronutrient for plants. Due to the requirement for Fe of the photosynthetic apparatus, the majority of shoot Fe content is localised in the chloroplasts of mesophyll cells. The reduction-based mechanism has prime importance in the
Julie Dardare et al.
Frontiers in oncology, 12, 1052163-1052163 (2022-12-27)
Damage specific DNA binding protein 2 (DDB2) is an UV-indiced DNA damage recognition factor and regulator of cancer development and progression. DDB2 has dual roles in several cancers, either as an oncogene or as a tumor suppressor gene, depending on
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