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5006

Sigma-Aldrich

PureCol

protein from bovine hide, suitable for cell culture

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EC Number:
NACRES:
NA.75

biological source

protein from bovine hide

Quality Level

sterility

sterile-filtered

Assay

99.9%

form

lyophilized

packaging

pkg of 15 mg

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

−20°C

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This Item
5074C42435133
Sigma-Aldrich

Sigma-Aldrich

5006

PureCol

Sigma-Aldrich

Sigma-Aldrich

5133

FibriCol®

sterility

sterile-filtered

sterility

sterile

sterility

sterile-filtered

sterility

sterile-filtered

assay

99.9%

assay

≥99% (SDS-PAGE Electrophoresis – Silver Stain, SDS-PAGE)

assay

≥99.9% (SDS-PAGE)

assay

>99% (SDS-PAGE Electrophoresis – Silver Stain, SDS-PAGE)

form

lyophilized

form

solid

form

liquid

form

solution

packaging

pkg of 15 mg

packaging

pkg of 35 mL

packaging

pkg of 100 mL, pkg of 20 mL

packaging

pkg of 20 mL

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

General description

PureCol collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol, lyophilized form is not recommended for the formation of a solid gel. PureCol collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Application

PureCol has been used:
  • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
  • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
  • to coat culture plates for collagen degradation assay with breast cancer cell lines

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

Costar is a registered trademark of Corning, Inc.
PureCol is a trademark of Advanced BioMatrix, Inc.
Transwell is a registered trademark of Corning, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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