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CFBE41o- Human CF Bronchial Epithelial Cell Line

CFBE41o- human CF bronchial epithelial cell line was derived from a cystic fibrosis patient homozygous for the ΔF508 CFTR mutation.

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Synonym(s):
CFBE41o, CF41o, CFBE
eCl@ss:
32011203

biological source

human

technique(s)

cell based assay: suitable
cell culture | mammalian: suitable

shipped in

ambient

Related Categories

General description

Cystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene which functions as a cAMP-activated and phosphorylated-regulated Cl channel. The predominant mutation in the CFTR gene is a trinucleotide deletion that results in loss of a phenylalanine at amino acids 508 (ΔF508) in the CFTR protein. This mutation accounts for ~66% of all CF alleles.

CFBE41o- is a CF human bronchial epithelial cell line, derived from a CF patient homozygous for the ΔF508 CFTR mutation and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) . CFBE14o- displays all ion transport properties characteristic of cystic fibrosis such as defective cAMP-dependent chloride transport and intact calcium-dependent chloride transport. Under appropriate culture conditions, CFBE41o- forms tight junctions to give a polarized epithelium .

The CFBE41o- cell line has also been used to generate subclones complemented with wild-type or ΔF508CFTR that possess differing levels of transgene-derived CFTR mRNA expression . These complemented CFBE41o- subclones (Cat. No. SCC158 – SCC161) suggest a direct correlation in CFTR mRNA expression levels to the number of active CFTR channels .

Cell Line Description

Epithelial Cells

Application

CFBE41o- human CF bronchial epithelial cell line was derived from a cystic fibrosis patient homozygous for the ΔF508 CFTR mutation.
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Disclaimer

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

6.2 - Infectious substances

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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K K Goncz et al.
British journal of pharmacology, 128(1), 227-233 (1999-09-28)
1. Exposure to epinephrine has been shown to have a range of effects on cells and tissues. A recent study suggested that the proliferative ability of CF epithelial cells, exposed to high concentrations of epinephrine (200 - 300 microM), was
E Bruscia et al.
Gene therapy, 9(11), 683-685 (2002-05-29)
Cystic fibrosis is the most common inherited disease in the Caucasian population. About 70% of all CF chromosomes carry the DeltaF508 mutation, a 3-bp deletion that results in the loss of a phenylalanine at amino acid 508 in the CF
Beate Illek et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 26(6), 983-990 (2011-01-12)
While the Cl(-) efflux assays are relatively straightforward, their ability to assess the efficacy of phenotypic correction in cystic fibrosis (CF) tissue or cells may be limited. Accurate assessment of therapeutic efficacy, i.e., correlating wild type CF transmembrane conductance regulator
Fanny Degrugillier et al.
International journal of molecular sciences, 21(14) (2020-07-12)
Cystic Fibrosis is a lethal monogenic autosomal recessive disease linked to mutations in Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein. The most frequent mutation is the deletion of phenylalanine at position 508 of the protein. This F508del-CFTR mutation leads to
Parameet Kumar et al.
Respiratory research, 20(1), 284-284 (2019-12-18)
Recent advances in the functional analyses of endogenous non-coding RNA (ncRNA) molecules, including long non-coding RNAs (LncRNAs), have provided a new perspective on the crucial roles of RNA in gene regulation. Consequently, LncRNA deregulation is a key factor in various

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