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5074

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PureCol EZ Gel solution

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Synonym(s):
Collagen type
NACRES:
NA.75

biological source

bovine skin

Quality Level

sterility

sterile

assay

≥99% (SDS-PAGE Electrophoresis – Silver Stain, SDS-PAGE)

form

solid

packaging

pkg of 35 mL

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

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This Item
C4243G1393C2124
vibrant-m

5074

PureCol EZ Gel solution

vibrant-m

C4243

Collagen solution from bovine skin

vibrant-m

G1393

Gelatin solution

vibrant-m

C2124

Collagen solution from bovine skin

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

≥99% (SDS-PAGE Electrophoresis – Silver Stain, SDS-PAGE)

assay

≥99.9% (SDS-PAGE)

assay

-

assay

-

biological source

bovine skin

biological source

bovine skin

biological source

bovine hide

biological source

bovine skin

sterility

sterile

sterility

sterile-filtered

sterility

sterile

sterility

sterile-filtered

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

PureCol is used in cell culture, tissue engineering and as a coating material for medical devices.PureCol EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I bovine collagen at a concentration of approximately 5 mg/ml (0.5%), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.

Application

The PureCol EZ Gel solution has been used:
  • for coating culture plates to facilitate the seeding and growth of epithelial cells derived from human nasal epithelial tissue
  • as a component in the bioartificial tendon preparation, providing a gel matrix for seeding human tendon cells and promoting their growth and collagen gel formation
  • for embedding mouse Achilles tendon-derived cells in 3D-collagen constructs, providing a biomimetic environment for studying tendon biology and performing qPCR, immunohistochemical, and western blot analyses.

Features and Benefits

PureCol EZ Gel is designed to improve gel consistency by providing a pre-formulated solution of media and collagen that have been adjusted to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions. PureCol EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution. PureCol EZ Gel is available in 35 mL volume, and produced by aseptic processing. 3D gels allow for the study of the effects of the mechanical properties of the ECM, such as density and rigidity, on cell development, migration, and morphology. Unlike 2D systems, 3D environments allow cell extensions to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways. Gel stiffness or rigidity also affects cell migration differently in 3D versus 2D environments. Furthermore, integrin-independent mechanical interactions resulting from the entanglement of matrix fibrils with cell extensions are possible in 3D systems, but not in 2D systems where the cells are attached to a flat surface.1-3

Preparation Note

3-D Gel Preparation Procedure
1. Remove PureCol EZ Gel from 2-10°C storage. To prevent gelation, maintain temperature of product at 2-10°C.
2. Introduce PureCol EZ Gel into cell culture system. Cells can be added to the PureCol EZ Gel solution.
3. To form gel, warm to 37°C. The beginning of gelation will occur within 40 minutes, but allow approximately 90 to minutes for firm gel formation.

Legal Information

PureCol is a trademark of Advanced BioMatrix, Inc.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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