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71183

Sigma-Aldrich

Sodium acetate

anhydrous, BioUltra, for luminescence, for molecular biology, ≥99.0% (NT)

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Synonym(s):
Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous, Acetic acid sodium salt
Linear Formula:
CH3COONa
CAS Number:
Molecular Weight:
82.03
Beilstein/REAXYS Number:
3595639
EC Number:
MDL number:
eCl@ss:
39021906
PubChem Substance ID:
NACRES:
NA.25

grade

for luminescence
for molecular biology

Quality Level

product line

BioUltra

assay

≥99.0% (NT)

form

solid

autoignition temp.

1112 °F

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

loss

≤0.5% loss on drying, 150 °C

pH

8.5-10.0 (25 °C, 3 M in H2O)

pKa 

4.76 (acetic acid)

mp

>300 °C (dec.) (lit.)

solubility

H2O: 3 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤20 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤20 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤10 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤10 mg/kg
Zn: ≤5 mg/kg

SMILES string

[Na+].CC([O-])=O

λ

3 M in H2O

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.02

InChI

1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1

InChI key

VMHLLURERBWHNL-UHFFFAOYSA-M

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1 of 4

This Item
S2889S754571188
vibrant-m

71183

Sodium acetate

Premium Grade
vibrant-m

S2889

Sodium acetate

-
vibrant-m

S7545

Sodium acetate

Premium Grade
vibrant-m

71188

Sodium acetate trihydrate

Premium Grade
grade

for luminescence, for molecular biology

grade

anhydrous, for molecular biology

grade

-

grade

-

product line

BioUltra

product line

-

product line

BioXtra

product line

BioUltra

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solid

form

solid

form

solid

form

powder or crystals

pH

8.5-10.0 (25 °C, 3 M in H2O)

pH

8.5-9.9 (25 °C, 246 g/L)

pH

8.5-9.9 (25 °C, 246 g/L)

pH

8.5-10.0 (25 °C, 3 M in H2O)

Application

Sodium acetate is suitable for use as a buffer in:
  • protein crystallization
  • staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
  • the preparation of standard (acetylcholine chloride) for hestrin assay
  • substrate screening characterization for microfluidic microbial fuel cell array

Biochem/physiol Actions

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Other Notes

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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