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84097

Sigma-Aldrich

Sucrose

BioUltra, for molecular biology, ≥99.5% (HPLC)

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Synonym(s):
α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
Empirical Formula (Hill Notation):
C12H22O11
CAS Number:
Molecular Weight:
342.30
Beilstein/REAXYS Number:
90825
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

product line

BioUltra

assay

≥99.5% (HPLC)

form

crystals

optical activity

[α]20/D +66.5±1°, c = 26% in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.01% (as SO4)

loss

≤0.1% loss on drying, 20 °C (HV)

color

white

pH

5.0-7.0 (25 °C, 1 M in H2O)

mp

185-187 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

SMILES string

OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

UV absorption

λ: 260 nm Amax: 0.12
λ: 280 nm Amax: 0.10

InChI

1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1

InChI key

CZMRCDWAGMRECN-UGDNZRGBSA-N

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1 of 4

This Item
S790384100573113
vibrant-m

84097

Sucrose

Premium Grade
vibrant-m

S7903

Sucrose

Premium Grade
vibrant-m

84100

Sucrose

-
vibrant-m

573113

Sucrose

-
assay

≥99.5% (HPLC)

assay

≥99.5% (GC)

assay

≥99.0% (HPLC)

assay

≥99%

grade

for molecular biology

grade

-

grade

ACS reagent

grade

Molecular Biology

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

H2O: 1 M, clear, colorless

solubility

H2O: 0.1 g/mL, clear, colorless

solubility

water: soluble

color

white

color

white

color

-

color

white

impurities

DNases, none detected, insoluble matter, passes filter test, proteases, none detected, RNases, none detected, phosphatases, none detected

impurities

Insoluble matter, passes filter test

impurities

≤0.0008 meq/g free acid, ≤0.005% water insoluble matter, ≤0.05% invert sugar, ≤5 mg/kg heavy metals (as Pb)

impurities

-

Application

Sucrose is a widely used nutitional disaccharide of glucose and fructose. Sucrose is used in molecular biology for the preparation of density gradients.

Other Notes

To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Isopycnic centrifugation in a discontinuous sucrose density gradient e.g. in the isolation of mRNA from membrane-bound polysomes

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Isolation of messenger RNA from membrane-bound polysomes.
B M Mechler
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