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Sigma-Aldrich

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

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Synonym(s):
Urate oxidase
CAS Number:
9002-12-4
Enzyme Commission number:
1.7.3.3 (BRENDA, IUBMB)
MDL number:
MFCD00082126
UNSPSC Code:
12352204
PubChem Substance ID:
57653596
NACRES:
NA.54

biological source

Bacillus sp. (B. fastidiosus)

Quality Level

300

form

powder

quality

lyophilized

specific activity

~9 U/mg

storage temp.

−20°C

SMILES string

N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

General description

Research area: Cell signaling
Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Application

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Biochem/physiol Actions

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Unit Definition

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Other Notes

For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Youssef M Roman
Journal of personalized medicine, 13(9) (2023-09-28)
Uric acid is the final product of purine metabolism and is converted to allantoin in most mammals via the uricase enzyme. The accumulation of loss of function mutations in the uricase gene rendered hominoids (apes and humans) to have higher
A Therapeutic Uricase with Reduced Immunogenicity Risk and Improved Development Properties
Nyborg AC, et al.
PLoS ONE (2016)
U. Naegele et al.
Methods of Enzymatic Analysis, 7, 140-140 (1985)
Berna Dalkıran et al.
Mikrochimica acta, 187(11), 609-609 (2020-10-16)
Electropolymerization of thionine (TH) on multiwalled carbon nanotube (MWCNT)-modified glassy carbon electrodes (GCE) in ethaline deep eutectic solvent (DES) was carried out for the first time, to prepare poly(thionine) (PTH) films with different nanostructured morphologies. PTH films were formed on
M.H. Jensen et al.
Methods of Enzymatic Analysis, 7, 101-101 (1985)
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