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S6639

Sigma-Aldrich

SSC Buffer 20× Concentrate

for Northern and Southern blotting, solution

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Synonym(s):
20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

description

Saline-Sodium Citrate buffer, made with ultrapure water

sterility

0.2 μm filtered

form

solution

application(s)

diagnostic assay manufacturing

foreign activity

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

storage temp.

room temp

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This Item
SRE0068S090293017
SSC Buffer 20× Concentrate for Northern and Southern blotting, solution

Sigma-Aldrich

S6639

SSC Buffer 20× Concentrate

Sigma-Aldrich

Sigma-Aldrich

SRE0068

SSC Buffer, 20X Concentrate

Sigma-Aldrich

Sigma-Aldrich

S0902

SSC Buffer

Sigma-Aldrich

Sigma-Aldrich

93017

SSC Buffer 20x

sterility

0.2 μm filtered

sterility

-

sterility

-

sterility

-

form

solution

form

liquid

form

powder blend

form

solution

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

-

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

foreign activity

-

storage temp.

room temp

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

General description

SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.

Application

SSC Buffer 20× Concentrate has been used
  • as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
  • as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
  • as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations

Components

SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
Liu H, et al.
Alzheimers Dement., 12(2), 130-143 (2016)
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
Chang-Gong Liu et al.
Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
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Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
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