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Y1376

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without leucine

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MDL number:
NACRES:
ND.02

Quality Level

form

powder

application(s)

food and beverages

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Y2146Y1771Y1896
application(s)

food and beverages

application(s)

microbiology

application(s)

microbiology

application(s)

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Components

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for leucine. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Quantity

1.6 g will supplement 1 liter of medium.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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1000309185

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Articles

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

Protocols

Yeast Drop Out

Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Although yeast can grow on a synthetic medium without any amino acids, better yield and growth rate can be achieved on richer media.

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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