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Y1376

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without leucine

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Synonym(s):
Synthetic Yeast Growth Media
MDL number:
UNSPSC Code:
12000000
NACRES:
ND.02

Quality Level

form

powder

application(s)

food and beverages

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application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Components

The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for leucine. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Quantity

1.6 g will supplement 1 liter of medium.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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