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A1603

Sigma-Aldrich

Alginate Lyase

powder, ≥10,000 units/g solid

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Synonym(s):
poly-beta-D-mannuronate lyase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

form

powder

Quality Level

specific activity

≥10,000 units/g solid

storage temp.

2-8°C

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This Item
C0897C6137P1016
Alginate Lyase powder, ≥10,000 units/g solid

Sigma-Aldrich

A1603

Alginate Lyase

specific activity

≥10,000 units/g solid

specific activity

≥0.20 unit/mg solid

specific activity

≥200 units/g solid

specific activity

0.8-2.0 units/mg protein

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

General description

Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.

Application

Alginate Lyase has been used:
  • in the pre-processing of enzyme
  • to de-encapsulate cells
  • to digest Pseudomonas exopolysaccharide to facilitate electroporation

Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture .

Biochem/physiol Actions

Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.
Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.

Unit Definition

One unit will produce an increase the A235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C

Preparation Note

Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

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Customers Also Viewed

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W Hashimoto et al.
Journal of bacteriology, 182(16), 4572-4577 (2000-07-27)
A bacterium, Sphingomonas sp. strain A1, can incorporate alginate into cells through a novel ABC (ATP-binding cassette) transporter system specific to the macromolecule. The transported alginate is depolymerized to di- and trisaccharides by three kinds of cytoplasmic alginate lyases (A1-I
Shinji Sakai et al.
Biomaterials, 33(28), 6721-6727 (2012-07-10)
We report a method for wrapping tissues with a pre-established cage-like layer composed of living cells. We encapsulated multicellular aggregates of human hepatoma HepG2 cells as a model of tissues such as pancreatic islets and hepatocyte spheroids in alginate-based hydrogel
Elizabeth M Parrish et al.
Reproduction (Cambridge, England), 142(2), 309-318 (2011-05-26)
Ovarian follicle maturation results from a complex interplay of endocrine, paracrine, and direct cell-cell interactions. This study compared the dynamic expression of key developmental genes during folliculogenesis in vivo and during in vitro culture in a 3D alginate hydrogel system.
François Thomas et al.
Environmental microbiology, 14(9), 2379-2394 (2012-04-20)
Alginate constitutes a significant part of seaweed biomass and thus a crucial nutrient for numerous marine heterotrophic bacteria. However, the mechanisms for alginate assimilation remain largely unknown in marine microorganisms. We show here that the genome of the marine flavobacterium
Bunzo Mikami et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 9), 1207-1216 (2012-09-06)
The structures of two mutants (H192A and Y246F) of a mannuronate-specific alginate lyase, A1-III, from Sphingomonas species A1 complexed with a tetrasaccharide substrate [4-deoxy-L-erythro-hex-4-ene-pyranosyluronate-(mannuronate)(2)-mannuronic acid] were determined by X-ray crystallography at around 2.2 Å resolution together with the apo form

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