A1603

Alginate Lyase

powder, ≥10,000 units/g solid

• Synonym(s): poly-beta-D-mannuronate lyase
• CAS Number: 9024-15-1
• Enzyme Commission number: 4.2.2.3 (BRENDA, IUBMB)
• MDL number: MFCD03454593
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: powder
• Quality Level: 200
• specific activity: ≥10,000 units/g solid
• storage temp.: 2-8°C

Description

General description

Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.[1]

Application

Alginate Lyase has been used:

• in the pre-processing of enzyme[2]
• to de-encapsulate cells[3]
• to digest Pseudomonas exopolysaccharide to facilitate electroporation[4]

Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture [5].

Biochem/physiol Actions

Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.[1]

Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.

Unit Definition

One unit will produce an increase the A_235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C

Preparation Note

Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)