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A4325

Sigma-Aldrich

Monoclonal Anti-α-Adaptin antibody produced in mouse

clone 100/2, ascites fluid

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Synonym(s):
Anti-AP-2
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

100/2, monoclonal

contains

15 mM sodium azide

species reactivity

bovine, human, rat

technique(s)

western blot: 1:200 using a crude preparation of synaptic vesicles obtained from rat cerebral cortex

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AP2A1(160)
rat ... Ap2a1(308578)

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This Item
A4450A7107SAB5701529
biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

species reactivity

bovine, human, rat

species reactivity

human, bovine, rat

species reactivity

human

species reactivity

rat, human, mouse

clone

100/2, monoclonal

clone

100/1, monoclonal

clone

A6/2/2, monoclonal

clone

monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

-

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General description

Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components.
Monoclonal Anti-a-Adaptin (AP-2) (mouse IgG2a isotype) is derived from the 100/2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptor related protein complex 2 subunit alpha 1 (AP2A1) codes for αA adaptin of the AP-2 complex. It is usually seen in clathrin coated vesicles.
The antibody reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma, or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody stains the intact α-adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of α-fragments obtained by trypsin cleavage of the α-subunit molecules.

Immunogen

AP-2 adaptor polypeptides from bovine brain.

Application

Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.
Monoclonal Anti-α-Adaptin antibody produced in mouse has been used in immunoblotting.
Mouse monoclonal anti-α-adaptin antibody has been used:
  • as a positive control in immunoblotting assays
  • as a primary antibody to detect AP2 levels in tetracycline-repressible stable cell lines

Biochem/physiol Actions

Adaptor related protein complex 2 subunit α 1 (AP2α1) is very essential for epidermal growth factor receptor (EGFR) nuclear translocation, induced by ultraviolet (UV).

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mark G H Scott et al.
The Journal of biological chemistry, 277(5), 3552-3559 (2001-10-17)
The process of clathrin-mediated endocytosis tightly regulates signaling of the superfamily of seven-transmembrane G protein-coupled receptors (GPCRs). A fundamental question in the cell biology of membrane receptor endocytosis is whether activated receptors can initiate the formation of clathrin-coated pits (CPs)
Vincent Blot et al.
Retrovirology, 3, 62-62 (2006-09-19)
Retrovirus particles emerge from the assembly of two structural protein components, Gag that is translated as a soluble protein in the cytoplasm of the host cells, and Env, a type I transmembrane protein. Because both components are translated in different
Juana M Del Valle et al.
The Journal of biological chemistry, 278(19), 17430-17437 (2003-03-07)
CD229 (Ly9) is a cell surface receptor selectively expressed on T and B lymphocytes, and it belongs to the CD150 receptor family. Like other receptors of this family, CD229 interacts with SAP/SH2D1a protein, mutation of which is responsible for the
Abstract 4049: AP2A1 is required for UV-induced EGFR nuclear translocation
Hsu SH
Cancer research, 4049-4049 (2011)
R A Warren et al.
The Journal of biological chemistry, 273(27), 17056-17063 (1998-06-27)
Endocytosis of surface proteins through clathrin-coated pits requires an internalization signal in the cytoplasmic domain. Two types of internalization signal have been described: one requiring a tyrosine as the critical residue (tyrosine-based motif), and the other consisting of either two

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