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A5633

Sigma-Aldrich

Antifoam A Concentrate

active silicone polymer 100 %

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NACRES:
NA.25

description

100% active silicone polymer

Quality Level

form

suspension

does not contain

emulsifier

composition

active silicone polymer, 100%

technique(s)

cell culture | mammalian: suitable
microbiological culture: suitable

General description

Antifoam A Concentrate is an extremely effective foam suppressor for aqueous and non-aqueous systems. It is 100% active silicone polymer. No emulsifiers are present. Antifoam A Concentrate is typically effective at 1-100 ppm. Antifoam A Concentrate should be diluted with 3-10 parts of propylene glycol (aqueous) or vegetable oil (nonaqueous) with slow mixing. The product will be stable in the pH range of 5 to 9. Antifoam A Concentrate can be added directly to a fermentation medium, but it is not recommended that it be pumped to a fermenter on an as-needed basis.

Application

An extremely effective foam suppresser for use in aqueous and non-aqueous systems.
Antifoam A Concentrate has been used as a component of Luria Bertani culture medium for growth of BL21 (DE3) E. coli cells transformed with the plasmids. It has also been used as a constituent in the preparation of aerosol generation fluid.

Reconstitution

1-50 ppm is adequate for most applications.

pictograms

Environment

signalword

Warning

hcodes

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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