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A6582

Sigma-Aldrich

Antifoam A concentrate

active silicone polymer 100%

NACRES:
NA.85

Quality Level

form

emulsion (aqueous)

technique(s)

cell culture | hybridoma: suitable
microbiological culture: suitable

density

0.97 g/mL at 25 °C

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

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This Item
A8011A5633A5757
Antifoam A concentrate active silicone polymer 100%

Sigma-Aldrich

A6582

Antifoam A concentrate

Antifoam C Emulsion aqueous-silicone emulsion

Sigma-Aldrich

A8011

Antifoam C Emulsion

Antifoam A Concentrate active silicone polymer 100 %

Sigma-Aldrich

A5633

Antifoam A Concentrate

Sigma-Aldrich

Sigma-Aldrich

A5757

Antifoam B Emulsion

technique(s)

cell culture | hybridoma: suitable, microbiological culture: suitable

technique(s)

cell culture | hybridoma: suitable, microbiological culture: suitable

technique(s)

cell culture | mammalian: suitable, microbiological culture: suitable

technique(s)

cell culture | hybridoma: suitable, microbiological culture: suitable

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

suitability

(Mammalian (suspension)), bacteria (fermentation)

suitability

-

suitability

-

suitability

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

density

0.97 g/mL at 25 °C

density

-

density

-

density

-

General description

Antifoam A is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. Extremely effective for aqueous and non-aqueous systems. Effective at concentrations in the 1-50ppm range.

Application

Antifoam A concentrate has been used:
  • in bioreactor culture for Staphylococcal enterotoxin C (SEC) production.
  • to prevent foam formation during the aerobic treatment of sludge.
  • to study the impact of surface tension on bioaerosol generation during bubbling.

Features and Benefits

  • Molecular biology grade and tested for use in bacterial fermentation.
  • Extremely effective foam suppressor for aqueous and non-aqueous systems.
  • Made of 100% active silicone polymer, free of emulsifiers.
  • Typically, effective at 1-100 ppm.
  • Product will be stable in the pH range of 5 to 9.
  • Can be directly added to a fermentation medium but it is not recommended that it be pumped to a fermenter on an as-needed basis.

Components

Antifoam A is a 100% active silicon polymer containing no non-ionic emulsifiers .

Preparation Note

Antifoam A can be pre-diluted with 3-10 parts of aqueous propylene glycol or non-aqueous vegetable oil with slow mixing.

Pictograms

Environment

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

213.8 °F - closed cup

Flash Point(C)

> 101 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Factors affecting staphylococcal enterotoxin C bovine production in milk
Hunt K, et al.
International dairy journal, 39(1), 41-46 (2014)
The biodegradability of aquatic worm predated waste activated sludge: A sequential aerobic and anaerobic treatment approach
de Valk S, et al.
bioresource technology reports, 12.0, 100606-100606 (2020)
Experience with a perfo-suction system for the recovery of schistosomes from laboratory rodents
T. F. H. G. Jackson, C. D. Dettman, et al.
Laboratory Animal Science, 16, 65-67 (1982)
Bioaerosol Release from Concentrated Microbial Suspensions in Bubbling Processes
Kruglyakova E, et al.
Air quality, atmosphere, & health, 13.0, 2029-2029 (2022)
M Schlömann et al.
Journal of bacteriology, 172(9), 5119-5129 (1990-09-01)
Enzymatic conversion of 4-fluorocatechol in the simultaneous presence of partially purified preparations of catechol 1,2-dioxygenase from Pseudomonas cepacia and muconate cycloisomerase from Alcaligenes eutrophus 335 yielded a product that was unambiguously identified as (+)-4-fluoromuconolactone [(+)-4-carboxymethyl-4-fluoro-but-2-en-4-olide]. This compound was shown to

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