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A8582

Sigma-Aldrich

Antifoam SE-15

aqueous emulsion for bacterial and mammalian systems

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Synonym(s):
silicone-based antifoam
NACRES:
NA.85

Quality Level

form

emulsion (aqueous)

concentration

10% (active silicon)

technique(s)

cell culture | hybridoma: suitable

solubility

H2O: soluble (effective hot or cold.)

density

1.0 g/mL at 25 °C

application(s)

microbiology

storage temp.

room temp

suitability

(Mammalian (suspension))
bacteria (fermentation)

General description

Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Application

Antifoam SE-15 has been used:

  • in the preparation of ramified rolling circle amplification (RAM) amplification buffer.
  • to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
  • as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay.
  • as a component of autoinduction expression culture.
  • as a component of cyclic adenosine monophosphate (cAMP) detection buffer.

Features and Benefits

  • Controls foaming in microbial culture flasks or fermentations
  • Non-toxic to the culture medium
  • Can be pumped to fermenter on an as-needed basis

Components

Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .

Preparation Note

Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.

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