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A8481

Sigma-Aldrich

Anti-γ-Actin antibody, Mouse monoclonal

clone 2-2.1.14.17, purified from hybridoma cell culture

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Synonym(s):
Anti-ACTG1
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

2-2.1.14.17, monoclonal

form

buffered aqueous solution

mol wt

antigen ~43 kDa

species reactivity

mouse, chicken, canine, bovine, human, hamster

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... ACTG1(404122)
chicken ... ACTG1(415296)
human ... ACTG1(71)
mouse ... Actg1(11465)

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This Item
SAB4200602A3853SAB1411364
species reactivity

mouse, chicken, canine, bovine, human, hamster

species reactivity

human, carp, bovine, frog, sheep, rabbit, rat, snake, guinea pig

species reactivity

-

species reactivity

human

clone

2-2.1.14.17, monoclonal

clone

5C5, monoclonal

clone

AC-40, monoclonal

clone

polyclonal

Gene Information

bovine ... ACTG1(404122)
chicken ... ACTG1(415296)
human ... ACTG1(71)
mouse ... Actg1(11465)

Gene Information

human ... ACTA1(58)

Gene Information

-

Gene Information

human ... ACTG2(72)

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

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General description

γ-Actin is mainly present in sarcolemma and to a lesser extent in reticular lattice of normal muscles. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.
Monoclonal Anti-γ-Actin (mouse IgG1 isotype) is derived from the hybridoma 2-2.1.14.17 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Actin isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 NH2-terminal residues. Actin constitutes for around 15% of the total protein. It exists in globular and filamentous forms.

Specificity

Monoclonal Anti-γ-Actin recognizes human, bovine, canine, mouse, hamster and chicken γ-actin.

Application

Anti-γ-Actin antibody, Mouse monoclonal has been used in :
  • immunofluorescence
  • enzyme linked immunosorbent assay (ELISA)
  • western blotting
  • immunohistochemistry

Mouse monoclonal Anti-γ-Actin antibody has been used for immunofluorescence assays. The antibody can also be used for ELISA, IHC and western blot assays.
Protein lysates from human Jurkat T-leukemic cells were subjected to western blot and probed using monoclonal mouse anti-actin (AC-40) to ensure equal loading.

Biochem/physiol Actions

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin is significantly involved in the microfilament system. It participates in muscle contraction and gene regulation. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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