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A9042

Sigma-Aldrich

Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit Anti-Dog IgG (whole molecule)−HRP

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About This Item

MDL number:
MFCD00162268
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:25,000
dot blot: 1:40,000 using direct chemiluminescence assay using 10 ng dog IgG/dot. Luminol plus enhancer was used as substrate.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Immunoglobulins (Igs) belong to immunoglobulin super-family. IgG is abundant immunoglobulin in proteins in human serum. The four classes of IgG can be IgG1, IgG2, IgG3 and IgG4.

Specificity

Anti-dog IgG antibody is specific for IgG in dogs.

Immunogen

Dog IgG

Application

Anti-Dog IgG (whole molecule)-peroxidase antibody has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • IgG/dot
  • western blotting

Biochem/physiol Actions

Dog IgG is a glycoprotein antibody that mediates intestinal antibacterial defense mechanisms. It may regulate the phagocytosis of Gram-negative bacteria . Anti-dog IgG antibody is specific for IgG in dogs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maria Agallou et al.
PloS one, 11(2), e0149894-e0149894 (2016-02-26)
Leishmania infantum is the etiologic agent of zoonotic visceral leishmaniasis (VL) in countries in the Mediterranean basin, where dogs are the domestic reservoirs and represent important elements in the transmission of the disease. Since the major focal areas of human
R J Heddle et al.
Immunology, 29(1), 197-208 (1975-07-01)
Secretory IgA antibodies to Vibrio cholerae were purified from the parotid saliva and mammary secretions of locally and orally immunized dogs using gel filtration, ion-exchange chromatography and anti-immunoglobulin immuno-absorbents. IgM and IgG antibodies were isolated from serum by gel filtration
Xiaohui Yao et al.
Frontiers in cellular and infection microbiology, 13, 1291937-1291937 (2024-01-18)
The Hedi virus (HEDV) and Wuxiang virus (WUXV) are newly discovered Bunyaviruses transmitted by sandflies. The geographical distribution of isolation of these two viruses continues to expand and it has been reported that WUXV causes neurological symptoms and even death
Characterization of the Major Antigenic Protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and Its Application for Serodiagnosis of Ehrlichiosis.
Knowles, T., et al.
Clinical and Vaccine Immunology : CVI, 10(4), 520-524 (2003)
IgG subclasses and allotypes: from structure to effector functions
Vidarsson G, et al.
Frontiers in Immunology, 5(2), 520-520 (2014)
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