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B6008

Sigma-Aldrich

Ponceau BS

Dye content ~60 %

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Synonym(s):
Acid Red 66, Biebrich scarlet WS, Biebrich scarlet sodium salt
Empirical Formula (Hill Notation):
C22H14N4Na2O7S2
CAS Number:
Molecular Weight:
556.48
Colour Index Number:
26905
Beilstein:
3883900
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

composition

Dye content, ~60%

solubility

ethanol: 1 mg/mL
H2O: >30 mg/mL

εmax

≥19000 at 510 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1ccc2ccccc2c1\N=N\c3ccc(cc3S([O-])(=O)=O)\N=N\c4ccc(cc4)S([O-])(=O)=O

InChI

1S/C22H16N4O7S2.2Na/c27-20-12-5-14-3-1-2-4-18(14)22(20)26-25-19-11-8-16(13-21(19)35(31,32)33)24-23-15-6-9-17(10-7-15)34(28,29)30;;/h1-13,27H,(H,28,29,30)(H,31,32,33);;/q;2*+1/p-2/b24-23+,26-25+;;

InChI key

VVAVKBBTPWYADW-RVTJCSDESA-L

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This Item
C8822P2395S4261
Ponceau BS Dye content ~60 %

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B6008

Ponceau BS

Crocein Scarlet 7B

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C8822

Crocein Scarlet 7B

Ponceau Xylidine Dye content ≥60 %

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P2395

Ponceau Xylidine

Sudan IV certified by the Biological Stain Commission, BioXtra

Sigma-Aldrich

S4261

Sudan IV

composition

Dye content, ~60%

composition

Dye content, ~70%

composition

Dye content, ≥60%

composition

Dye content, ≥80%

solubility

ethanol: 1 mg/mL, H2O: >30 mg/mL

solubility

H2O: 1 mg/mL

solubility

water: 10 mg/mL, clear, red

solubility

-

εmax

≥19000 at 510 nm

εmax

-

εmax

18-22 at 503-508 nm in water

εmax

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

Ponceau BS has been used in Masson′s trichrome stain for the staining of collagen. It has also been used for eosinophil staining.

Biochem/physiol Actions

Ponceau BS is used in Masson′s trichrome staining procedure, along with acid fuchsin, for the labeling of collagen and muscle red.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Interleukin-4 has basophilic and eosinophilic cell growth-promoting activity on cord blood cells.
Favre C, et al.
Blood, 75, 67-67 (1990)
Shariff S and Kaler AK
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We studied the non-specific interactions of two azo compounds: biebrich scarlet (BS) and naphthochrome green (NG), with four model proteins: bovine serum albumin, ovalbumin, poly-L-lysine and hemoglobin by UV-VIS spectrometry, fluorophotometry and circular dichroism melting technique. The optimal acidities of
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(2), 253-257 (2003-01-21)
Here we describe a simple histochemical technique that provides an improved approach to identifying eosinophil components in tissues through the formation of photoreactive complexes that produce stable fluorescent emissions. This method worked readily with histological tissue sections 6-60 microm thick

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