C0255
Collagenase from Clostridium histolyticum
purified by chromatography, ≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder
Synonym(s):
Clostridiopeptidase A
About This Item
Recommended Products
form
lyophilized powder
specific activity
≥500 CDU/mg solid (CDU = collagen digestion units)
mol wt
68-130 kDa
purified by
chromatography
solubility
TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C
foreign activity
Clostripain ≤2.0 U/mg
storage temp.
−20°C
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Related Categories
General description
Application
- for the recovery of neutrophils from collagen gels
- for the dissociation of breast tumor biopsies into single cells
- for the isolation of primary human endometrial stromal cells(HESC) from endometrial biopsies
Biochem/physiol Actions
Packaging
Unit Definition
Preparation Note
Substrate
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificates of Analysis (COA)
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Protocols
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
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