MilliporeSigma
All Photos(2)

Documents

C2581

Sigma-Aldrich

Anti-Cholecystokinin (26-33) (CCK-8) antibody produced in rabbit

whole antiserum

Sign Into View Organizational & Contract Pricing

Synonym(s):
CCK Antibody - Anti-Cholecystokinin (26-33) (CCK-8) antibody produced in rabbit, Cck Antibody
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

competitive inhibition ELISA: 1:1000-1:2000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:8,000 using indirect immunoperoxidase staining of human stomach

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CCK(885)

General description

Cholecystokinin (CCK) belongs to gastrointestinal hormone family is a neuropeptide hormone and neurotransmitter usually present in gastrointestinal (GI) tract and the central nervous system (CNS). CCK functions include enzyme secretion from pancreas, gall bladder contraction, intestinal motility; CCK will also inhibit gastrin induced acid secretion . This product is also useful for the study of mode of action, differential tissue expression and intracellular and subcellular localization of CCK in CNS and in neuroendocrine cells of digestive system. Anti-CCK-8 reacts specifically with sulfated CCK-8 and shows cross reactivity with unsulfated CCK-8 and Big Gastrin I.
Cholecystokinin (CCK) is a neuropeptide hormone and neurotransmitter widely distributed throughout the gastrointestinal (GI) tract and the central nervous system (CNS). CCK, Big Gastrin I, and vasoactive intestinal peptide (VIP) belong to the gastrin/cholcystokinin family. hormone family. CCK has a biologically active C-terminal pentapeptide. CCK exists as a larger precursor hormone, preproCCK (114 amino acids), from which several smaller fragments are derived, sharing a C-terminal tetrapeptide and a sulfated tyrosine residue. Sulfated CCK (26-33) amide (CCK-8) is the major and the most potent CCK form in the brain and periphery. CCK is widely distributed in several brain regions, including the cerebral cortex, hippocampus, amygdala nuclei, and the hypothalamus. In the periphery, CCK is localized mainly in nerve fibers in the myenteric and submucosal ganglia, as well as in endocrine cells of the gastrointestinal tract.

Immunogen

Synthetic sulfated CCK-8 conjugated to KLH.

Application

Anti-Cholecystokinin (26-33) (CCK-8) has been used in

  • immunohistochemistry
  • double-label immunofluorescence staining
  • immunocytochemistry
  • ELISA immunoassay
Anti-cholecystokinin (26-33) (CCK-8) antibody can be used to detect CCK peptide in pancreatic cancer cells by immunocytochemistry. It may also be used for ELISA immunoassay.
Anti-cholecystokinin (26-33) (CCK-8) antibody can be used to detect CCK peptide in pancreatic cancer cells by immunocytochemistry. It may also be used for dot blot immunoassay.

Biochem/physiol Actions

Cholecystokinin (CCK) stimulates enzyme secretion from the pancreas, gall bladder contraction, and intestinal motility and it inhibits gastrin-induced acid secretion. CCK may be involved in several physiological and behavioral functions such as satiety, anxiety, memory processes, and analgesia and in disorders such as panic disorder. In the central nervous system (CNS), CCK acts as a neurotransmitter and modulates the action of other neurotransmitters such as dopamine, 5-hydroxytryptamine (5-HT), γ-Aminobutyric acid (GABA) and excitatory amino acids. The multiple biological actions of CCK are mediated by two classes of receptors, the peripheral/brain CCK-A receptors and the brain CCK-B receptors.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service