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MilliporeSigma

C3175

Chloramphenicol-Water Soluble

powder, suitable for cell culture, BioReagent

Synonym(s):

Chloramphenicol, Chlornitromycin, Chlorocid, Chloromycetin, Cloramfenicol, D-(−)-threo-2-Dichloroacetamido-1-(4-nitrophenyl)-1,3-propanediol, D-(−)-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-β-(4-nitrophenyl)ethyl]acetamide, Chloromycetin, D-threo-2,2-Dichloro-N-[β-hydroxy-α-(hydroxymethyl)-4-nitrophenethyl]aceta, Kloramfenikol

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.76
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Product Name

Chloramphenicol-Water Soluble, powder, BioReagent, suitable for cell culture

product line

BioReagent

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 50-500 mg/mL (stock solution), PBS: 50-500 mg/mL (stock solution), other salt solutions: not recommended

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, mycobacteria, mycoplasma

mode of action

protein synthesis | interferes

Application

Chloramphenicol-Water Soluble has been used:

  • as a supplement in Luria-Bertani (LB) medium to culture Escherichia coli
  • as a supplement in LB agar to culture Citrobacter rodentium
  • to culture Toxoplasma gondii

Biochem/physiol Actions

Chloramphenicol is a natural product found in Streptomyces venezuelae. It shows a broad range of antibiotic activity against Gram-negative and Gram-positive bacteria by binding to the peptidyl transferase loop of the ribosomal RNA to block the peptide bond formation.

Packaging

Package size based on chloramphenicol

Other Notes

Formulated to contain approx. 100 mg chloramphenicol per gram of powder; balance 2-hydroxypropyl-β-cyclodextrin.
Keep container tightly closed in a dry and well-ventilated place. Light sensitive. Keep in a dry place.


pictograms

Health hazardCorrosion

signalword

Danger

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Repr. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges



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Articles

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic


A Chatzitakis et al.
Water research, 42(1-2), 386-394 (2007-08-19)
The photocatalytic degradation of Chloramphenicol, an antibiotic drug, has been investigated in aqueous heterogeneous solutions containing n-type oxide semiconductors as photocatalysts. The disappearance of the organic molecule follows approximately a pseudo-first-order kinetics according to the Langmuir-Hinshelwood model. It was observed
D H Burke et al.
Chemistry & biology, 4(11), 833-843 (1998-01-31)
The problem of how macromolecules adopt specific shapes to recognize small molecules in their environment is readily addressed through in vitro selections (the SELEX protocol). RNA-antibiotic interactions are particularly attractive systems for study because they provide an opportunity to expand
Jaap C Hanekamp et al.
Environmental toxicology and pharmacology, 39(1), 213-220 (2014-12-22)
The antibiotic chloramphenicol (CAP) is banned from food production. Besides being a medicinal product, CAP is also a natural product, produced by Streptomyces Venezuelae. The lack of scientific data hampers setting of an Acceptable Daily Intake (ADI). Consequently, a maximum



Global Trade Item Number

SKUGTIN
C3175-100MG04061833485552