C4231
Anti-phospho-β-Catenin (pSer33/pSer37) antibody, Mouse monoclonal
clone BC-22, purified from hybridoma cell culture
Synonym(s):
Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo
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About This Item
Pricing and availability is not currently available.
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BC-22, monoclonal
form
buffered aqueous solution
mol wt
antigen ~94 kDa
species reactivity
mouse, human, rat, chicken
concentration
~2 mg/mL
General description
CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The encoded protein β-catenin is localized to the nucleus.
Specificity
The antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphosphorylated peptide. It does not detect the unphosphorylated or the Ser33 phosphorylated protein and does not recognize phosphorylated plakoglobin.
Immunogen
synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human β-catenin.
Application
Monoclonal Anti-phospho-ß-Catenin (pSer33/pSer37) antibody is suitable for:
- indirect ELISA
- microarray[1]
- western blot at a dilution of 5-10μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132.
Biochem/physiol Actions
β-catenin is mapped on the short arm of chromosome 3. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and hence can be significant in the CCA therapy target.
In Wnt/β-catenin signal pathway β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The protein encoded has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression colon cancer progression.
In Wnt/β-catenin signal pathway β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The protein encoded has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression colon cancer progression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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SOX7 Suppresses Wnt Signaling by Disrupting β-Catenin/BCL9 Interaction.
Fan R, et al.
Dna and Cell Biology, ;37(2), 126-132 (2018)
Localization of the human beta-catenin gene (CTNNB1) to 3p21: a region implicated in tumor development.
Kraus C, et al.
Genomics, 23(1), 272-274 (1994)
Nuclear α-catenin mediates the DNA damage response via β-catenin and nuclear actin.
Leonid A S, et al.
Journal of Cell Science, 130, 1717-1729 (2017)
β-catenin is a valuable marker for differential diagnosis of osteoblastoma and osteosarcoma.
Wan Y, et al.
Human Pathology, 45(7), 1459-1465 (2014)
Simon Megy et al.
FEBS letters, 580(22), 5411-5422 (2006-09-26)
The interaction of the P-beta-Cat(19-44) peptide, a 26 amino acid peptide (K(19)AAVSHWQQQSYLDpSGIHpSGATTTAP(44)) that mimics the phosphorylated beta-Catenin antigen, has been studied with its monoclonal antibody BC-22, by transferred nuclear Overhauser effect NMR spectroscopy (TRNOESY) and saturation transfer difference NMR (STD
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